Clinical and biologic features of the HOXA-expressing T-ALL cases
. | . | . | . | . | Chromosomal rearrangements . | . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Case ID . | Sex . | Age, y . | WBC count, × 109/L . | Immunophenotype . | MLL . | CALM-AF10 . | HOXA . | CDKN2A deletion . | ||
| TL36 | M | 4 | 290 | γδ | MLL-AF9 | — | — | ND | ||
| TL37 | F | 16 | 199 | i | MLL-ENL | — | — | — | ||
| TL38 | M | 5 | 41 | i | MLL-ENL | — | — | — | ||
| TL39 | M | 3 | 147 | γδ | — | CALM-AF10 | — | — | ||
| TL40 | M | 17 | 296 | γδ | — | CALM-AF10 | — | + | ||
| TL41 | M | 4 | 215 | γδ | — | CALM-AF10 | — | + | ||
| TL42 | F | 48 | 112 | i | — | CALM-AF10 | — | — | ||
| TL43 | F | 25 | 85 | αβ | — | — | TCRB-HOXA | + | ||
| TL44 | M | 9 | 412 | γδ | — | — | TCRB-HOXA | + | ||
| TL45 | F | 43 | 159 | αβ | — | — | TCRB-HOXA | + | ||
| TL46 | M | 13 | 17 | αβ | — | — | TCRB-HOXA | — | ||
| TL47 | F | 29 | 109 | γδ | — | — | —* | + | ||
| TL48 | M | 39 | 245 | γδ | — | — | —* | + | ||
. | . | . | . | . | Chromosomal rearrangements . | . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Case ID . | Sex . | Age, y . | WBC count, × 109/L . | Immunophenotype . | MLL . | CALM-AF10 . | HOXA . | CDKN2A deletion . | ||
| TL36 | M | 4 | 290 | γδ | MLL-AF9 | — | — | ND | ||
| TL37 | F | 16 | 199 | i | MLL-ENL | — | — | — | ||
| TL38 | M | 5 | 41 | i | MLL-ENL | — | — | — | ||
| TL39 | M | 3 | 147 | γδ | — | CALM-AF10 | — | — | ||
| TL40 | M | 17 | 296 | γδ | — | CALM-AF10 | — | + | ||
| TL41 | M | 4 | 215 | γδ | — | CALM-AF10 | — | + | ||
| TL42 | F | 48 | 112 | i | — | CALM-AF10 | — | — | ||
| TL43 | F | 25 | 85 | αβ | — | — | TCRB-HOXA | + | ||
| TL44 | M | 9 | 412 | γδ | — | — | TCRB-HOXA | + | ||
| TL45 | F | 43 | 159 | αβ | — | — | TCRB-HOXA | + | ||
| TL46 | M | 13 | 17 | αβ | — | — | TCRB-HOXA | — | ||
| TL47 | F | 29 | 109 | γδ | — | — | —* | + | ||
| TL48 | M | 39 | 245 | γδ | — | — | —* | + | ||
Chromosomal rearrangements were investigated by MLL and HOXA FISH and Southern blot, and by CALM-AF10 RT-PCR. MLL partners were further identified in MLL-rearranged cases as described in “Materials and methods.” In addition, deletion of the CDKN2A (CDKN2A/INK4A/ARF) gene was analyzed by FISH.
WBC indicates peripheral white blood cells; M, male; γδ, TCRγδ expression; —, no rearrangement detected; ND, not done; F, female; i, immature immunophenotype; +, CDKN2A deletion; αβ, TCRαβ expression.
These cases were classified as HOXA-related considering their HOXA dysregulated pattern and frequent coclustering with the TCRB-HOXA rearranged cases