Inhibition of agonist-induced PAC1 binding by Sema3A
Agonist . | Concentration (% inhibition) . |
|---|---|
| ADP, μM | 5 (90.4 ± 12.1) |
| PAR1-TRAP, μM | 15 (115.2 ± 10.2) |
| Thrombin, U/mL | 0.5 (97.4 ± 3.3) |
| U46619, μM | 2 (112.5 ± 9.3) |
| Convulxin, ng/mL | 5 (94.7 ± 6.0) |
| A23187, μM | 2.5 (106 ± 5.5) |
| PMA, nM | 200 (58.1 ± 14.3) |
Agonist . | Concentration (% inhibition) . |
|---|---|
| ADP, μM | 5 (90.4 ± 12.1) |
| PAR1-TRAP, μM | 15 (115.2 ± 10.2) |
| Thrombin, U/mL | 0.5 (97.4 ± 3.3) |
| U46619, μM | 2 (112.5 ± 9.3) |
| Convulxin, ng/mL | 5 (94.7 ± 6.0) |
| A23187, μM | 2.5 (106 ± 5.5) |
| PMA, nM | 200 (58.1 ± 14.3) |
Platelets preincubated with 10 μg/mL Sema3A/Fc were activated with indicated agonists, and FITC-PAC1 binding was detected as demonstrated in Figure 2. Percent inhibition of mean fluorescent intensity against hIgG-treated platelets was calculated. Data represent mean ± SE of at least 3 independent experiments.