Erythrocyte levels of zinc protoporphyrin and free protoporphyrin IX are markedly increased in IRP2-/- and IRP1+/- IRP2-/- animals
. | IRP1+/+ IRP2+/+ . | IRP1+/+ IRP2+/- . | IRP1+/+ IRP2-/- . | IRP1+/- IRP2-/- . |
|---|---|---|---|---|
| Zn protoporphyrin, μM | 0.7 ± 0.15 | 0.74 ± 0.05 | 2.84 ± 1.34 | 4.87 ± 1.25 |
| Free protoporphyrin, μM | 0.21 ± 0.07 | 0.27 ± 0.02 | 41.97 ± 14.12 | 40.80 ± 5.70 |
. | IRP1+/+ IRP2+/+ . | IRP1+/+ IRP2+/- . | IRP1+/+ IRP2-/- . | IRP1+/- IRP2-/- . |
|---|---|---|---|---|
| Zn protoporphyrin, μM | 0.7 ± 0.15 | 0.74 ± 0.05 | 2.84 ± 1.34 | 4.87 ± 1.25 |
| Free protoporphyrin, μM | 0.21 ± 0.07 | 0.27 ± 0.02 | 41.97 ± 14.12 | 40.80 ± 5.70 |
Levels of erythrocyte free and zinc protoporphyrin IX in washed peripheral RBCs were measured as described in materials and methods. Levels of Zn protoporphyrin were significantly elevated in IRP2-/- animals (P = .01) and IRP1+/- IRP2-/- animals (P = .007). Free protoporphrin IX levels were markedly elevated in IRP2-/- animals (P < .001) and IRP1+/- IRP2-/- animals (P < .001). Data presented as mean values ± standard deviation.