Iron in parts per million from tissue ashing and organ weights of spleen and liver in WT, IRP2-/-, and IRP1+/- IRP2-/- animals reveals increased liver iron content
. | IRP1+/+ IRP2+/+ . | IRP1+/+ IRP2-/- . | IRP1+/- IRP2-/- . |
|---|---|---|---|
| Iron content, ppm | |||
| Brain | 102 ± 13 | 108 ± 5 | 93 ± 13 |
| Spleen | 1852 ± 697 | 1554 ± 388 | 1841 ± 635 |
| Liver | 360 ± 72 | 748 ± 204 | 1279 ± 342 |
| Organ weights, g | |||
| Spleen | 0.10 ± 0.04 | 0.08 ± 0.01 | 0.04 ± 0.05 |
| Liver | 1.47 ± 0.49 | 1.39 ± 0.38 | 0.99 ± 0.22 |
| Body | 39 ± 9 | 39 ± 9 | 23 ± 5 |
. | IRP1+/+ IRP2+/+ . | IRP1+/+ IRP2-/- . | IRP1+/- IRP2-/- . |
|---|---|---|---|
| Iron content, ppm | |||
| Brain | 102 ± 13 | 108 ± 5 | 93 ± 13 |
| Spleen | 1852 ± 697 | 1554 ± 388 | 1841 ± 635 |
| Liver | 360 ± 72 | 748 ± 204 | 1279 ± 342 |
| Organ weights, g | |||
| Spleen | 0.10 ± 0.04 | 0.08 ± 0.01 | 0.04 ± 0.05 |
| Liver | 1.47 ± 0.49 | 1.39 ± 0.38 | 0.99 ± 0.22 |
| Body | 39 ± 9 | 39 ± 9 | 23 ± 5 |
Ashing and determination of iron content as described in materials and methods revealed a statistically significant increase in liver iron in IRP1+/- IRP2-/- animals compared with WT (P = .038), and a significant decrease in body weight compared to WT (P = .004). Data presented as mean values ± standard deviation.