Table 3. Percentages of trisomy 8 cells... | American Society of Hematology

Table 3.

Percentages of trisomy 8 cells in BMMC cultures after short-term culture

		CD3d-BM + autologous Vβ-expanded CD8, %					MHC class 1 mAb, %
Patient	BM alone, no treatment, %	Direct cell contact	Separated by filter	Direct cell contact + Fas antagonist	mAb alone	mAb + autologous Vβ-expanded CD8	Matched	Mismatched
32	16	1	15	5	18	15	18	—
15	35	4	30	14	22	28	29	—
21	78	24	68	40	68	70	72	74

		CD3d-BM + autologous Vβ-expanded CD8, %					MHC class 1 mAb, %
Patient	BM alone, no treatment, %	Direct cell contact	Separated by filter	Direct cell contact + Fas antagonist	mAb alone	mAb + autologous Vβ-expanded CD8	Matched	Mismatched
32	16	1	15	5	18	15	18	—
15	35	4	30	14	22	28	29	—
21	78	24	68	40	68	70	72	74

Samples were cocultured as described in “Patients, materials, and methods.” BM was T cell depleted using beads before cocultivation with lymphocytes or mAb. Purified expanded Vβ subfamilies were obtained by flow cytometric sorting. When lymphocytes were separated from BMMCs by a filter, the inhibitory effect of Vβ lymphocytes was not apparent, suggesting a dependence on direct cell-to-cell contact. Fas antagonist (1 μg/mL) partially blocked the effect of CD8 cells on trisomy 8, suggesting the importance of Fas-mediated apoptosis in the elimination of trisomy 8 cells. HLA class 1 mAb (1:20) also partially blocked the effect of expanded CD8 cells when it was incubated for 30 minutes with the lymphocyte-depleted BMMCs before the addition of lymphocytes. Allogeneic HLA class 1-mismatched CD8 cells, which would presumably be activated after cocultivation with BMMCs, did not decrease the proportion of trisomy 8 cells when they were added in 3-fold excess to autologous lymphocytes.

— indicates not done.

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