Reproducibility of RQ-PCR
. | LPL . | . | ADAM29 . | . | ||
|---|---|---|---|---|---|---|
| Patient . | Ct ± SD . | CV, % . | Ct ± SD . | CV, % . | ||
| CLL-73 | 23.54 ± 0.46 | 1.97 | NA | NA | ||
| CLL-105 | 23.96 ± 0.38 | 1.59 | NA | NA | ||
| CLL-67 | NA | NA | 27.33 ± 1.18 | 4.31 | ||
| CLL-101 | NA | NA | 23.32 | 2.21 | ||
. | LPL . | . | ADAM29 . | . | ||
|---|---|---|---|---|---|---|
| Patient . | Ct ± SD . | CV, % . | Ct ± SD . | CV, % . | ||
| CLL-73 | 23.54 ± 0.46 | 1.97 | NA | NA | ||
| CLL-105 | 23.96 ± 0.38 | 1.59 | NA | NA | ||
| CLL-67 | NA | NA | 27.33 ± 1.18 | 4.31 | ||
| CLL-101 | NA | NA | 23.32 | 2.21 | ||
Overall variability of RQ-PCR quantification of LPL and ADAM29 gene expression was evaluated on 12 replicates performed on 4 different CLL patients. This was done by testing each sample in 4 replicates during the same reaction (intrarun variability), this being repeated on 3 different days (interrun variability).
Ct indicates threshold cycle; SD, standard deviation; CV, coefficient of variation; NA, not applicable.