Murine PCR primers
Gene . | Sequence . | Size, bp . | Annealing temperature, °C . |
|---|---|---|---|
| IL-3Rα | 636 | 60 | |
| Sense | 5′-TGC ACT ACC GGA TGT TCT GG-3′ | ||
| Antisense | 5′-ACT TCC TCC ACC ACA GCA GG-3′ | ||
| ALP | 517 | 60 | |
| Sense | 5′-AAC CCA GAC ACA AGC ATT CC-3′ | ||
| Antisense | 5′-GAG ACA TTT TCC CGT TCA CC-3′ | ||
| OC | 430 | 60 | |
| Sense | 5′-GCA GCT TGG TGC ACA CCT AG-3′ | ||
| Antisense | 5′-GGA GCT GCT GTG ACA TCC ATA C-3′ | ||
| Runx2 | 308 | 60 | |
| Sense | 5′-CCC AGC CAC CTT TAC CTA CA-3′ | ||
| Antisense | 5′-CAG CGT CAA CAC CAT CAT TC-3′ | ||
| GAPDH | 451 | 60 | |
| Sense | 5′-ACC ACA GTC CAT GCC ATC AC-3′ | ||
| Antisense | 5′-TCC ACC ACC CTG TTG CTG TA-3′ |
Gene . | Sequence . | Size, bp . | Annealing temperature, °C . |
|---|---|---|---|
| IL-3Rα | 636 | 60 | |
| Sense | 5′-TGC ACT ACC GGA TGT TCT GG-3′ | ||
| Antisense | 5′-ACT TCC TCC ACC ACA GCA GG-3′ | ||
| ALP | 517 | 60 | |
| Sense | 5′-AAC CCA GAC ACA AGC ATT CC-3′ | ||
| Antisense | 5′-GAG ACA TTT TCC CGT TCA CC-3′ | ||
| OC | 430 | 60 | |
| Sense | 5′-GCA GCT TGG TGC ACA CCT AG-3′ | ||
| Antisense | 5′-GGA GCT GCT GTG ACA TCC ATA C-3′ | ||
| Runx2 | 308 | 60 | |
| Sense | 5′-CCC AGC CAC CTT TAC CTA CA-3′ | ||
| Antisense | 5′-CAG CGT CAA CAC CAT CAT TC-3′ | ||
| GAPDH | 451 | 60 | |
| Sense | 5′-ACC ACA GTC CAT GCC ATC AC-3′ | ||
| Antisense | 5′-TCC ACC ACC CTG TTG CTG TA-3′ |