Percentages of hematopoietic stem and progenitor cells in PU.1 nonconditional and conditional knockout mice
. | . | Positive percent for each fraction . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype . | Age . | HSC . | CLP . | CMP . | GMP . | MEP . | Granulocyte . | Monocyte . | ||||||
| PU.1-/- | E14.5 | 0.036* | <0.01* | <0.01* | <0.01* | 3.98 | <0.01* | 0.05* | ||||||
| PU.1-/+ | E14.5 | 0.41 | 0.16 | 0.66 | 0.88 | 3.96 | 0.72 | 2.98 | ||||||
| PU.1+/+ | E14.5 | 0.43 | 0.35 | 0.75 | 1.21 | 3.50 | 1.08 | 6.48 | ||||||
| Mx1-Cre+PU.1F/F | 2 wk | 0.10 | <0.01* | 0.012* | 0.032* | 2.33* | 2.49* | 4.07 | ||||||
| PU.1F/F (Cre-) | 2 wk | 0.12 | 0.068 | 0.75 | 0.37 | 0.90 | 7.14 | 3.58 | ||||||
| Mx1-Cre+ PU.1F/F | 4 wk | 0.51 | <0.01* | 0.027* | 0.027* | 14.71* | 3.07 | 9.42 | ||||||
| PU.1F/F (Cre-) | 4 wk | 0.35 | 0.11 | 0.75 | 0.59 | 1.44 | 7 | 9.55 | ||||||
| Mx1-Cre+ PU.1F/F | 6 wk | 0.22 | 0.076* | 0.16* | 0.88 | 2.33* | 10.25 | 11.48 | ||||||
| PU.1F/F (Cre-) | 6 wk | 0.29 | 0.19 | 0.74 | 1.43 | 1.08 | 8.98 | 8.17 | ||||||
. | . | Positive percent for each fraction . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype . | Age . | HSC . | CLP . | CMP . | GMP . | MEP . | Granulocyte . | Monocyte . | ||||||
| PU.1-/- | E14.5 | 0.036* | <0.01* | <0.01* | <0.01* | 3.98 | <0.01* | 0.05* | ||||||
| PU.1-/+ | E14.5 | 0.41 | 0.16 | 0.66 | 0.88 | 3.96 | 0.72 | 2.98 | ||||||
| PU.1+/+ | E14.5 | 0.43 | 0.35 | 0.75 | 1.21 | 3.50 | 1.08 | 6.48 | ||||||
| Mx1-Cre+PU.1F/F | 2 wk | 0.10 | <0.01* | 0.012* | 0.032* | 2.33* | 2.49* | 4.07 | ||||||
| PU.1F/F (Cre-) | 2 wk | 0.12 | 0.068 | 0.75 | 0.37 | 0.90 | 7.14 | 3.58 | ||||||
| Mx1-Cre+ PU.1F/F | 4 wk | 0.51 | <0.01* | 0.027* | 0.027* | 14.71* | 3.07 | 9.42 | ||||||
| PU.1F/F (Cre-) | 4 wk | 0.35 | 0.11 | 0.75 | 0.59 | 1.44 | 7 | 9.55 | ||||||
| Mx1-Cre+ PU.1F/F | 6 wk | 0.22 | 0.076* | 0.16* | 0.88 | 2.33* | 10.25 | 11.48 | ||||||
| PU.1F/F (Cre-) | 6 wk | 0.29 | 0.19 | 0.74 | 1.43 | 1.08 | 8.98 | 8.17 | ||||||
For conditional disruption of floxed PU.1 alleles, pl-pC (single dose of 125 μg) was intraperitoneally injected in Mx1-Cre+ or Mx1-Cre- PU.1F/F mice at 1 to 2 days after birth.
P < .05.