Sequences of primers and probes
. | Primers . |
|---|---|
| cDNA cloning | |
| CF1 | 5′ CAA GAA TTC AGG AAT CAT CAA GGA ATG CAC ACT |
| CR1 | 5′ TCC TCT AGA CTG ACT CCA GCA ATA GTG GTG ATA TAT T |
| RT-PCR | |
| CF2 | 5′ ATG TGA ACA TGG AAT CAT CAA GGA ATG |
| CR2 | 5′ AGT TGA ACT TTC TGT TCT GC |
| TaqMan PCR | |
| TF1 | 5′ ATC CAG ATC TAA CTT GGG GTG G |
| TR1 | 5′ TGT GCT TTC TGC ATG TTT TCT GT |
| P1 (probe) | 5′ TTT GTC TTC TTC TTT TGC CAA TTC CAC TAA T — FAM |
| TF2 | 5′ GCA CAC TCA CCA GCA ACA CC |
| TR2 (=TR1) | 5′ TGT GCT TTC TGC ATG TTT TCT GT |
| P2 (probe) | 5′ AGT GCA AAG AGG AAG TGA AGA GAA AGG AAG — FAM |
| Genomic | |
| GF1 | 5′ GGC CCC TAA TTT ACA AAG TGC CA |
| GF2 | 5′ CCA ACC CCA TGG AAA GAT GTG |
| GR1 | 5′ GTT CTG AGA AGG GAA AAT TGA ATA CT |
| GR2 | 5′ CGC TAA AGG CAA GGC AAA CTT |
. | Primers . |
|---|---|
| cDNA cloning | |
| CF1 | 5′ CAA GAA TTC AGG AAT CAT CAA GGA ATG CAC ACT |
| CR1 | 5′ TCC TCT AGA CTG ACT CCA GCA ATA GTG GTG ATA TAT T |
| RT-PCR | |
| CF2 | 5′ ATG TGA ACA TGG AAT CAT CAA GGA ATG |
| CR2 | 5′ AGT TGA ACT TTC TGT TCT GC |
| TaqMan PCR | |
| TF1 | 5′ ATC CAG ATC TAA CTT GGG GTG G |
| TR1 | 5′ TGT GCT TTC TGC ATG TTT TCT GT |
| P1 (probe) | 5′ TTT GTC TTC TTC TTT TGC CAA TTC CAC TAA T — FAM |
| TF2 | 5′ GCA CAC TCA CCA GCA ACA CC |
| TR2 (=TR1) | 5′ TGT GCT TTC TGC ATG TTT TCT GT |
| P2 (probe) | 5′ AGT GCA AAG AGG AAG TGA AGA GAA AGG AAG — FAM |
| Genomic | |
| GF1 | 5′ GGC CCC TAA TTT ACA AAG TGC CA |
| GF2 | 5′ CCA ACC CCA TGG AAA GAT GTG |
| GR1 | 5′ GTT CTG AGA AGG GAA AAT TGA ATA CT |
| GR2 | 5′ CGC TAA AGG CAA GGC AAA CTT |
Positions are marked in Figure 1. Forty-two more primers were used for complete genome sequencing (not shown).