Phenotypic Cell Composition of Adult BM and of Adult and NBB
| Cells From . | Age . | Mean Percentage of Positive Cells ± SE . | |||||
|---|---|---|---|---|---|---|---|
| CD4+CD8− . | CD4−CD8+ . | CD4+CD8+ . | TCRα/βhigh . | Thy-1+ . | SCA-1+ . | ||
| Adult blood | 2-3 months | 16.2 ± 1.1 | 7.1 ± 1.0 | ND | 20.4 ± 1.8 | 17.7 ± 2.7 | 0.4 ± 0.1 |
| Newborn blood | Day 0 | 3.3 ± 0.7 | 2.3 ± 0.4 | 40.8 ± 8.4 | 3.0 ± 0.4 | 46.3 ± 8.3 | 17.0 ± 5.2 |
| Day 2 | 9.9 ± 2.3* | 5.0 ± 2.2 | 36.2 ± 6.9 | 6.9 ± 0.9* | 54.3 ± 6.0 | 9.0 ± 6.0 | |
| Day 5 | 13.2 ± 1.9* | 5.8 ± 1.7 | 20.1 ± 5.6* | 12.8 ± 2.7* | 36.0 ± 8.2† | 16.3 ± 3.9 | |
| Adult bone marrow | 2-3 months | 3.1 ± 0.3 | 2.0 ± 0.2 | 0.2 ± 0.1 | 7.8 ± 1.0 | 2.2 ± 0.1 | 12.5 ± 1.0 |
| Cells From . | Age . | Mean Percentage of Positive Cells ± SE . | |||||
|---|---|---|---|---|---|---|---|
| CD4+CD8− . | CD4−CD8+ . | CD4+CD8+ . | TCRα/βhigh . | Thy-1+ . | SCA-1+ . | ||
| Adult blood | 2-3 months | 16.2 ± 1.1 | 7.1 ± 1.0 | ND | 20.4 ± 1.8 | 17.7 ± 2.7 | 0.4 ± 0.1 |
| Newborn blood | Day 0 | 3.3 ± 0.7 | 2.3 ± 0.4 | 40.8 ± 8.4 | 3.0 ± 0.4 | 46.3 ± 8.3 | 17.0 ± 5.2 |
| Day 2 | 9.9 ± 2.3* | 5.0 ± 2.2 | 36.2 ± 6.9 | 6.9 ± 0.9* | 54.3 ± 6.0 | 9.0 ± 6.0 | |
| Day 5 | 13.2 ± 1.9* | 5.8 ± 1.7 | 20.1 ± 5.6* | 12.8 ± 2.7* | 36.0 ± 8.2† | 16.3 ± 3.9 | |
| Adult bone marrow | 2-3 months | 3.1 ± 0.3 | 2.0 ± 0.2 | 0.2 ± 0.1 | 7.8 ± 1.0 | 2.2 ± 0.1 | 12.5 ± 1.0 |
Adult bone marrow cells and peripheral blood cells from adult or newborn B10.D2 mice were stained with anti-CD4 and anti-CD8, anti-TCRα/β, anti–SCA-1 or anti–Thy-1 MoAb. Fluorescence of 10,000 cells was measured. Five individual mice were analyzed and mean percentage of positive cells ± SE was calculated.
Abbreviation: ND, not detected.
Significantly different when compared to newborn blood day 0 (Student’s t test P < .05).