Table 2. Sequential Differential Count... | American Society of Hematology

Table 2.

Sequential Differential Count of Recipient and Donor Cells as a Percentage According to Cell Type

Patient No. 1 CD	BM wk −2		Blood wk 3		Blood wk 7		Blood wk 9		BM wk 11		Blood wk 11		Blood wk 13		Blood wk 16
Patient No. 1 CD	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0
15			99.3^*	0.7	100	0	98	2^†	15.4^‡	84.6	46.0	54.0	0	100^‡	0	100
									(6)	(33)				(62)
3	13.3	86.6	4.3^*	95.7	11.5	88.5			1.4^*	98.6	0.5	99.5			0	100
	(10)	(65)
22	2.0	98.0	11.3^*	87.7	0	100			6.7^*	93.3					0.9	99.1
	(1)	(49)	(11)^‡	(85)^‡		(25)^‡
34	81.4^‡	19.6							12.8^‡	87.3
									(11)	(82)
GlyA	94.3^‡	5.7							1.9^*	98.1
Patient No. 2 CD	BM wk −8		Blood wk −8		Blood wk 6		Blood wk 8		Blood wk 10		Blood wk 13		Blood wk 17		Blood wk 21
Patient No. 2 CD	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX
15			100	0	98.0	2.0	91.8	8.2	96.5	3.5	96.8	3.2	0	100	0	100
																(14)
3	3.0	97.0	7.5	92.5	0	100	5.4	94.6	0.8	99.2	1.9	98.1	2.9	97.1
22	0	100	4.0	96.0	0	100	0	100	5.0	95.0			1.0	99.0
		(19)						(61)
34	90.0	10.0	88.3	11.7							88.5	12.5
							(5)	(2)			(56)	(8)		(2)
Gly	93.2	6.8
	(82)	(6)
Patient No. 3 CD	BM wk 0		Blood wk 0		Blood wk 5		Blood wk 7		Blood wk 9		Blood wk 12
Patient No. 3 CD	XY	XX	XY	XX	XY	XX	XY	XX	XY	XX	XY	XX
15	61.0	39.0	67.6	30.9	69.7	25.4	6.0	94.0	0	100	0	100
							(3)	(47)		(87)
3	1.9	98.1							0.6	99.4	0	100
22	0.8	99.2					0		2.2	97.8	0	100
34	8.0	92.0					0	100^2-153	0.9	99.1^2-153
								(18)
GlyA	11.5	88.5							0	100
										(18)

Patient No. 1 CD	BM wk −2		Blood wk 3		Blood wk 7		Blood wk 9		BM wk 11		Blood wk 11		Blood wk 13		Blood wk 16
Patient No. 1 CD	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0
15			99.3^*	0.7	100	0	98	2^†	15.4^‡	84.6	46.0	54.0	0	100^‡	0	100
									(6)	(33)				(62)
3	13.3	86.6	4.3^*	95.7	11.5	88.5			1.4^*	98.6	0.5	99.5			0	100
	(10)	(65)
22	2.0	98.0	11.3^*	87.7	0	100			6.7^*	93.3					0.9	99.1
	(1)	(49)	(11)^‡	(85)^‡		(25)^‡
34	81.4^‡	19.6							12.8^‡	87.3
									(11)	(82)
GlyA	94.3^‡	5.7							1.9^*	98.1
Patient No. 2 CD	BM wk −8		Blood wk −8		Blood wk 6		Blood wk 8		Blood wk 10		Blood wk 13		Blood wk 17		Blood wk 21
Patient No. 2 CD	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX	X0	XX
15			100	0	98.0	2.0	91.8	8.2	96.5	3.5	96.8	3.2	0	100	0	100
																(14)
3	3.0	97.0	7.5	92.5	0	100	5.4	94.6	0.8	99.2	1.9	98.1	2.9	97.1
22	0	100	4.0	96.0	0	100	0	100	5.0	95.0			1.0	99.0
		(19)						(61)
34	90.0	10.0	88.3	11.7							88.5	12.5
							(5)	(2)			(56)	(8)		(2)
Gly	93.2	6.8
	(82)	(6)
Patient No. 3 CD	BM wk 0		Blood wk 0		Blood wk 5		Blood wk 7		Blood wk 9		Blood wk 12
Patient No. 3 CD	XY	XX	XY	XX	XY	XX	XY	XX	XY	XX	XY	XX
15	61.0	39.0	67.6	30.9	69.7	25.4	6.0	94.0	0	100	0	100
							(3)	(47)		(87)
3	1.9	98.1							0.6	99.4	0	100
22	0.8	99.2					0		2.2	97.8	0	100
34	8.0	92.0					0	100^2-153	0.9	99.1^2-153
								(18)
GlyA	11.5	88.5							0	100
										(18)

Unshaded columns correspond to recipient values and shaded columns correspond to donor values. Vertical rows show cell types defined by positivity for different clusters of differentiation (CD). Results are expressed as the percentage of antigen-positive cells counted. In parentheses are the absolute numbers of counted cells in as less than 100.

Abbreviations: X0/XX, male/female cells defined by FISH using an X-specific probe; XY/XX, male/female cells defined by FISH using an X- and a Y-specific probe simultaneously; GlyA, glycophorin A; BM, bone marrow.

All female cells are XX1515.

†

Confirmed by simultaneous hybridization of an X- and a Y-specific probe.

‡

Week −2: 6% to 7% of female cells are XX15−; week 11: 1/11 CD34⁺, 1/6 CD15⁺ female cells are XX15−.

F2-153

Partial overlap with GlyA⁺ cells, as morphologically circulating erythroblasts − confirmed by MGG count.

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