Table 1.

Analysis of CNBr Fragments of rEC

Fragment Sequence Mass (kD)
Observed Calculated
−DTT +DTT
1  YVEFPR-V610 to M660  5  6  6.462 
2  D661 to M764  18  18  11.649  
3  Q765 to M839 7  8.5  8.642  
4  K840 to Q847  1  0.954 
Fragment Sequence Mass (kD)
Observed Calculated
−DTT +DTT
1  YVEFPR-V610 to M660  5  6  6.462 
2  D661 to M764  18  18  11.649  
3  Q765 to M839 7  8.5  8.642  
4  K840 to Q847  1  0.954 

CNBr cleavage of rαEC was performed as described in Materials and Methods. The fragments were analyzed on 16.5% Tris-Tricine SDS-PAGE (Bio-Rad) in the presence and absence of 0.1 mol/L DTT. Fragment sizes were determined by comparison with low-range SDS-PAGE standards from Bio-Rad. Sequence numbering is according to the full αE sequence (GenBank accession no. M58569).

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