Time Course of Cell Number, CD56 Expression, Viability, Percentage of Apoptosis, and Cell Cycle Status of CD34+HPCs Cultured in FL or KL for 21 Days, Followed by the Addition of IL-15 for 14 Days
| Day of Culture . | FL → FL + IL-15 . | KL → KL + IL-15 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of Cells/Well (×1,000) . | % CD56+ . | % Nonviable . | % Apoptosis . | % Cycling . | No. of Cells/Well (×1,000) . | % CD56+ . | % Nonviable . | % Apoptosis . | % Cycling . | |
| Day 21 | 154 ± 22 | 0.8 ± .05 | 2.5 ± 0.4 | 21 ± 8 | 14 ± 2 | 170 ± 28 | 0.7 ± .03 | 2.2 ± 0.5 | 13 ± 1 | 13 ± 2 |
| Day 24 | 171 ± 15 | 9.5 ± 2.2 | 2.6 ± 0.5 | 14 ± 3 | 12 ± 3 | 186 ± 20 | 3.7 ± 0.9 | 1.8 ± 0.4 | 14 ± 3 | 12 ± 1 |
| Day 27 | 163 ± 21 | 13 ± 2.8 | 5.7 ± 1.2 | 15 ± 2 | 10 ± 2 | 170 ± 22 | 11 ± 3.1 | 5.8 ± 1.0 | 10 ± 2 | 14 ± 3 |
| Day 30 | 135 ± 18 | 27 ± 2.5 | 6.3 ± 0.9 | 12 ± 2 | 12 ± 4 | 170 ± 18 | 25 ± 4.3 | 5.1 ± 0.6 | 11 ± 2 | 11 ± 3 |
| Day 33 | 138 ± 10 | 59 ± 6.4 | 9.8 ± 1.5 | 11 ± 2 | 14 ± 3 | 165 ± 17 | 54 ± 9.6 | 8.3 ± 0.7 | 12 ± 1 | 15 ± 1 |
| Day 36 | 138 ± 11 | 94 ± 3.1 | 8.6 ± 1.2 | 14 ± 1 | 11 ± 1 | 168 ± 15 | 83 ± 2.6 | 16 ± 2.1 | 13 ± 1 | 12 ± 2 |
| Day of Culture . | FL → FL + IL-15 . | KL → KL + IL-15 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of Cells/Well (×1,000) . | % CD56+ . | % Nonviable . | % Apoptosis . | % Cycling . | No. of Cells/Well (×1,000) . | % CD56+ . | % Nonviable . | % Apoptosis . | % Cycling . | |
| Day 21 | 154 ± 22 | 0.8 ± .05 | 2.5 ± 0.4 | 21 ± 8 | 14 ± 2 | 170 ± 28 | 0.7 ± .03 | 2.2 ± 0.5 | 13 ± 1 | 13 ± 2 |
| Day 24 | 171 ± 15 | 9.5 ± 2.2 | 2.6 ± 0.5 | 14 ± 3 | 12 ± 3 | 186 ± 20 | 3.7 ± 0.9 | 1.8 ± 0.4 | 14 ± 3 | 12 ± 1 |
| Day 27 | 163 ± 21 | 13 ± 2.8 | 5.7 ± 1.2 | 15 ± 2 | 10 ± 2 | 170 ± 22 | 11 ± 3.1 | 5.8 ± 1.0 | 10 ± 2 | 14 ± 3 |
| Day 30 | 135 ± 18 | 27 ± 2.5 | 6.3 ± 0.9 | 12 ± 2 | 12 ± 4 | 170 ± 18 | 25 ± 4.3 | 5.1 ± 0.6 | 11 ± 2 | 11 ± 3 |
| Day 33 | 138 ± 10 | 59 ± 6.4 | 9.8 ± 1.5 | 11 ± 2 | 14 ± 3 | 165 ± 17 | 54 ± 9.6 | 8.3 ± 0.7 | 12 ± 1 | 15 ± 1 |
| Day 36 | 138 ± 11 | 94 ± 3.1 | 8.6 ± 1.2 | 14 ± 1 | 11 ± 1 | 168 ± 15 | 83 ± 2.6 | 16 ± 2.1 | 13 ± 1 | 12 ± 2 |
Purified CD34+ HPCs were plated at a density of 2 × 104 cells per well and first cultured in medium containing FL or KL (100 ng/mL) for 21 days. IL-15 was then added to culture in combination with either FL or KL, respectively, for an additional 14 days. Cells were examined at 3-day intervals after the addition of IL-15, as described in Materials and Methods.