Table 2. Molecular Analysis of T-Cell... | American Society of Hematology

Table 2.

Molecular Analysis of T-Cell Clonality

Patient No.	Samples	Histology	TCR Status
Complicated CD
1	Duodenum and jejunum	Consistent with untreated CD	C (Vγ3-Jγ)^*
	MLN/Accessory spleen	Normal	C (Vγ3-Jγ)^*
2	Duodenum	Consistent with untreated CD	C (VγI-Jγ)
	Jejunum (108 days later)	Ulcerative jejunitis	C (VγI-Jγ)
3	Duodenum	Consistent with untreated CD	C (VγI-Jγ; Vγ9-Jγ)^*
	Colon (30 months later)	Small lymphocyte infiltration	C (VγI-Jγ; Vγ9-Jγ)^*
	MLN (30 months later)	Small lymphocyte infiltration	C (VγI-Jγ)
4	Duodenum	Consistent with untreated CD + ulcerations	C (Vγ8-Jγ)^*
	Jejunum (30 days later)	Consistent with untreated CD	C (Vγ8-Jγ)^*
	Peripheral blood (64 days later)	Large-cell lymphoma	C (Vγ8-Jγ)^*
Untreated CD responsive to GFD
5	Duodenum	Consistent with untreated CD	P
6	Duodenum	Consistent with untreated CD	P
7	Duodenum	Consistent with untreated CD	P
8	Duodenum	Consistent with untreated CD	P
9	Duodenum	Consistent with untreated CD	P
10	Duodenum	Consistent with untreated CD	P
11	Duodenum	Consistent with untreated CD	O (faint bands)
Controls
12	Duodenum	Normal villous architecture	P
13	Duodenum	Normal villous architecture	O (strong bands)
14	Duodenum	Normal villous architecture	O (faint bands)
15	Duodenum	Normal villous architecture	P

Patient No.	Samples	Histology	TCR Status
Complicated CD
1	Duodenum and jejunum	Consistent with untreated CD	C (Vγ3-Jγ)^*
	MLN/Accessory spleen	Normal	C (Vγ3-Jγ)^*
2	Duodenum	Consistent with untreated CD	C (VγI-Jγ)
	Jejunum (108 days later)	Ulcerative jejunitis	C (VγI-Jγ)
3	Duodenum	Consistent with untreated CD	C (VγI-Jγ; Vγ9-Jγ)^*
	Colon (30 months later)	Small lymphocyte infiltration	C (VγI-Jγ; Vγ9-Jγ)^*
	MLN (30 months later)	Small lymphocyte infiltration	C (VγI-Jγ)
4	Duodenum	Consistent with untreated CD + ulcerations	C (Vγ8-Jγ)^*
	Jejunum (30 days later)	Consistent with untreated CD	C (Vγ8-Jγ)^*
	Peripheral blood (64 days later)	Large-cell lymphoma	C (Vγ8-Jγ)^*
Untreated CD responsive to GFD
5	Duodenum	Consistent with untreated CD	P
6	Duodenum	Consistent with untreated CD	P
7	Duodenum	Consistent with untreated CD	P
8	Duodenum	Consistent with untreated CD	P
9	Duodenum	Consistent with untreated CD	P
10	Duodenum	Consistent with untreated CD	P
11	Duodenum	Consistent with untreated CD	O (faint bands)
Controls
12	Duodenum	Normal villous architecture	P
13	Duodenum	Normal villous architecture	O (strong bands)
14	Duodenum	Normal villous architecture	O (faint bands)
15	Duodenum	Normal villous architecture	P

Parentheses indicate the oligonucleotides with which monoclonal rearrangements were demonstrated.

The Vγ segments used were determined using Vγ specific PCR experiments and confirmed by sequencing.

Abbreviations: C, monoclonal rearrangements; P, polyclonal pattern; O, oligoclonal pattern; MLN, mesenteric lymph nodes.