Recovery of FVIII-neutralizing activity upon affinity purification
| Source of IgG . | Loaded activity (BU/mg) . | Recovered activity (BU/mg) . | Relative increase in activity . |
|---|---|---|---|
| Alloantibody HA | 1.0 | 57.0 | 57.0 |
| 18.0 | 57.0 | 3.2 | |
| 2.4 | 64.0 | 26.4 | |
| 22.4 | 43.0 | 1.9 | |
| Autoantibody | 9.6 | 10.0 | 1.0 |
| nd | nd | nd | |
| Nonresponder HA | 0 | 0 | 0 |
| 0 | 0 | 0 | |
| 0 | 0 | 0 | |
| Pooled nonresponder HA | 0 | 0.8† | — |
| Healthy donor | 0 | 0 | 0 |
| IVIg | 0 | 15.0 | — |
| Source of IgG . | Loaded activity (BU/mg) . | Recovered activity (BU/mg) . | Relative increase in activity . |
|---|---|---|---|
| Alloantibody HA | 1.0 | 57.0 | 57.0 |
| 18.0 | 57.0 | 3.2 | |
| 2.4 | 64.0 | 26.4 | |
| 22.4 | 43.0 | 1.9 | |
| Autoantibody | 9.6 | 10.0 | 1.0 |
| nd | nd | nd | |
| Nonresponder HA | 0 | 0 | 0 |
| 0 | 0 | 0 | |
| 0 | 0 | 0 | |
| Pooled nonresponder HA | 0 | 0.8† | — |
| Healthy donor | 0 | 0 | 0 |
| IVIg | 0 | 15.0 | — |
See Table 1 for description of IgG sources.
HA indicates patients with severe hemophilia A; nd, not done.
Pool of affinity-purified anti-FVIII IgG of the 3 nonresponder HA patients.