Table 5. Comparison of frequency of... | American Society of Hematology

Table 5.

Comparison of frequency of mutation of bcl-6 and β-globin genes in chronic lymphocytic leukemia

Patient^5-150	bcl-6					β-Globin^5-151
Patient^5-150	PCR	Number of clones sequenced	Total bp	Number of mutations (% clones mutated)	Frequency of mutations (bp⁻¹)	PCR	Number of clones sequenced	Total bp	Number of mutations (% clones mutated)	Frequency of mutations (bp⁻¹)
AG	PCR 1	6	4392	6 (67)	13.7 × 10⁻⁴	PCR 1	15	10 860	3 (13)	2.8 × 10⁻⁴
	PCR 2	10	7320	8 (75)	10.9 × 10⁻⁴
					mean 12.3 × 10⁻⁴
DB	PCR 1	5	3660	4 (80)	10.9 × 10⁻⁴	PCR 1	12	8688	2 (17)	2.3 × 10⁻⁴
	PCR 2	9	6588	5 (44)	7.6 × 10⁻⁴
					mean 9.3 × 10⁻⁴
AW	PCR 1	4	2928	0	0.0	ND
	PCR 2	4	2928	1 (25)	3.4 × 10⁻⁴
					mean 1.7 × 10⁻⁴

Patient^5-150	bcl-6					β-Globin^5-151
Patient^5-150	PCR	Number of clones sequenced	Total bp	Number of mutations (% clones mutated)	Frequency of mutations (bp⁻¹)	PCR	Number of clones sequenced	Total bp	Number of mutations (% clones mutated)	Frequency of mutations (bp⁻¹)
AG	PCR 1	6	4392	6 (67)	13.7 × 10⁻⁴	PCR 1	15	10 860	3 (13)	2.8 × 10⁻⁴
	PCR 2	10	7320	8 (75)	10.9 × 10⁻⁴
					mean 12.3 × 10⁻⁴
DB	PCR 1	5	3660	4 (80)	10.9 × 10⁻⁴	PCR 1	12	8688	2 (17)	2.3 × 10⁻⁴
	PCR 2	9	6588	5 (44)	7.6 × 10⁻⁴
					mean 9.3 × 10⁻⁴
AW	PCR 1	4	2928	0	0.0	ND
	PCR 2	4	2928	1 (25)	3.4 × 10⁻⁴
					mean 1.7 × 10⁻⁴

PCR = Polymerase chain reaction; bp = base pairs; ND = not determined.

Patients AG and DB from CLL (UM) subset, and patient AW from CLL (M) subset.

Taq error rate calculated from frequency of mutation of β-globin gene (mean from 2 determinations = 2.6 × 10⁻⁴bp⁻¹).