Reduced numbers of DC in the spleen, thymus, and LN of flt3L−/− mice
| . | flt3L+/+ CD11c++ CD8α− . | flt3L−/− CD11c++ CD8α− . | Fold reduction . | flt3L+/+ CD11c++ CD8α+ . | Flt3L−/− CD11c++ CD8α+ . | Fold reduction . |
|---|---|---|---|---|---|---|
| Spleen* | ||||||
| Experiment 1 (n = 3) | 17.8 | 1.6 | 11.1 | 10.0 | 0.7 | 14.3 |
| Experiment 2 (n = 4) | 17.5 | 1.9 | 9.2 | 4.7 | 0.4 | 11.8 |
| Experiment 3 (n = 3) | 12.8 | 3.1 | 4.1 | 5.1 | 0.8 | 6.4 |
| Thymus† | ||||||
| Experiment 1 (n = 5) | 6.9 | 0.9 | 7.7 | 9.0 | 1.2 | 7.5 |
| Experiment 2 (n = 8) | 8.0 | 0.9 | 8.9 | 8.7 | 1.7 | 5.1 |
| Experiment 3 (n = 4) | 12.2 | 4.6 | 2.7 | 11.7 | 2.4 | 4.9 |
| Experiment 4 (n = 3) | 3.9 | 1.4 | 2.8 | 4.9 | 0.8 | 6.1 |
| LN† | ||||||
| Experiment 1 (n = 5) | 4.2 | 1.5 | 2.8 | 5.5 | 0.6 | 9.2 |
| Experiment 2 (n = 8) | 1.6 | 0.2 | 8.0 | 1.2 | 0.3 | 4.0 |
| Experiment 3 (n = 4) | 1.9 | <0.1 | 1.2 | <0.1 | ||
| Experiment 4 (n = 5) | 1.7 | <0.1 | 1.3 | <0.1 | ||
| Experiment 5 (n = 7) | 2.6 | 0.2 | 13.0 | 1.8 | <0.1 |
| . | flt3L+/+ CD11c++ CD8α− . | flt3L−/− CD11c++ CD8α− . | Fold reduction . | flt3L+/+ CD11c++ CD8α+ . | Flt3L−/− CD11c++ CD8α+ . | Fold reduction . |
|---|---|---|---|---|---|---|
| Spleen* | ||||||
| Experiment 1 (n = 3) | 17.8 | 1.6 | 11.1 | 10.0 | 0.7 | 14.3 |
| Experiment 2 (n = 4) | 17.5 | 1.9 | 9.2 | 4.7 | 0.4 | 11.8 |
| Experiment 3 (n = 3) | 12.8 | 3.1 | 4.1 | 5.1 | 0.8 | 6.4 |
| Thymus† | ||||||
| Experiment 1 (n = 5) | 6.9 | 0.9 | 7.7 | 9.0 | 1.2 | 7.5 |
| Experiment 2 (n = 8) | 8.0 | 0.9 | 8.9 | 8.7 | 1.7 | 5.1 |
| Experiment 3 (n = 4) | 12.2 | 4.6 | 2.7 | 11.7 | 2.4 | 4.9 |
| Experiment 4 (n = 3) | 3.9 | 1.4 | 2.8 | 4.9 | 0.8 | 6.1 |
| LN† | ||||||
| Experiment 1 (n = 5) | 4.2 | 1.5 | 2.8 | 5.5 | 0.6 | 9.2 |
| Experiment 2 (n = 8) | 1.6 | 0.2 | 8.0 | 1.2 | 0.3 | 4.0 |
| Experiment 3 (n = 4) | 1.9 | <0.1 | 1.2 | <0.1 | ||
| Experiment 4 (n = 5) | 1.7 | <0.1 | 1.3 | <0.1 | ||
| Experiment 5 (n = 7) | 2.6 | 0.2 | 13.0 | 1.8 | <0.1 |
DC were enriched from the spleen, the thymus, and 6 LN (2 inguinal plus 2 brachial plus 2 axillary) as described in “Materials and methods.” Tissues were digested with collagenase, and the low-density cells were isolated by centrifugation on a Nycodenz gradient. The proportion of myeloid-related DC (CD11c++, CD8α−) and lymphoid-related DC (CD11c++, CD8α+) were analyzed by flow cytometry. The data are presented as the absolute number of DC per spleen, per thymus, or per 6 LN, from 3 to 5 experiments in which tissues from 3 to 8 mice were pooled for analysis.
×105.
×104.