TBARS, total lipid hydroperoxide and conjugated diene formation in a low density lipoprotein exposed to hematin or heme arginate
| Parameter . | 0 h . | 18 h . | 36 h . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| LDL . | LDL + Hematin . | LDL + HA . | LDL . | LDL + Hematin . | LDL + HA . | LDL . | LDL + Hematin . | LDL + HA . | |
| TBARS (nmol/mg LDL) | 0.07 ± 0.01 | 0.10 ± 0.02 | 0.10 ± 0.01 | 0.09 ± 0.03 | 10.48 ± 1.27 | 0.25 ± 0.09 | 0.12 ± 0.03 | 8.69 ± 1.61 | 11.07 ± 2.08 |
| Total LOOH (nmol/mg LDL) | 9.5 ± 0.2 | 9.0 ± 0.7 | 9.8 ± 0.1 | 8.0 ± 0.2 | 140.7 ± 2.5 | 9.7 ± 0.2 | 7.5 ± 1.8 | 156.9 ± 8.2 | 165.1 ± 15.6 |
| Conjugated dienes (Absorbance at 234 nm) | 0.245 ± 0.004 | 0.276 ± 0.006 | 0.289 ± 0.001 | 0.260 ± 0.002 | 1.058 ± 0.002 | 0.282 ± 0.003 | 0.258 ± 0.007 | 1.120 ± 0.011 | 1.150 ± 0.047 |
| Parameter . | 0 h . | 18 h . | 36 h . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| LDL . | LDL + Hematin . | LDL + HA . | LDL . | LDL + Hematin . | LDL + HA . | LDL . | LDL + Hematin . | LDL + HA . | |
| TBARS (nmol/mg LDL) | 0.07 ± 0.01 | 0.10 ± 0.02 | 0.10 ± 0.01 | 0.09 ± 0.03 | 10.48 ± 1.27 | 0.25 ± 0.09 | 0.12 ± 0.03 | 8.69 ± 1.61 | 11.07 ± 2.08 |
| Total LOOH (nmol/mg LDL) | 9.5 ± 0.2 | 9.0 ± 0.7 | 9.8 ± 0.1 | 8.0 ± 0.2 | 140.7 ± 2.5 | 9.7 ± 0.2 | 7.5 ± 1.8 | 156.9 ± 8.2 | 165.1 ± 15.6 |
| Conjugated dienes (Absorbance at 234 nm) | 0.245 ± 0.004 | 0.276 ± 0.006 | 0.289 ± 0.001 | 0.260 ± 0.002 | 1.058 ± 0.002 | 0.282 ± 0.003 | 0.258 ± 0.007 | 1.120 ± 0.011 | 1.150 ± 0.047 |
Low density lipoprotein (20 mg/dL protein) in NaCl (150)-HEPES (10) buffer (pH 7.4) was exposed to hematin (5 μM) or heme arginate (HA) (5 μM) for the indicated time periods in air. Lipid peroxidation was analyzed by measuring thiobarbituric acid-reactive substances (TBARS), total lipid hydroperoxide (LOOH), and conjugated dienes as described under “Materials and methods.” Results represent mean ± SE of 3 experiments performed in duplicate.