Table 1. Phenotyping population... | American Society of Hematology

Table 1.

Phenotyping population definitions

Cell population	Phenotype	References
B cells
CD19⁺ B cells	CD19⁺ lymphocytes	26-29
Nonswitched memory B cells	CD27⁺ IgD⁺ % of CD19⁺ B cells)	26
Naive B cells	CD38⁻CD10⁻ B cells (% of CD19⁺ B cells)	27
Mature naive B cells	IgD⁺CD27⁻CD19⁺	26
CD21^low B cells	CD19⁺CD21^low CD10^+/− / CD19⁺ B cells	28
Transitional B cells
T2 transitional B cells	CD38^intCD10^int IgD⁺CD27⁻ CD19⁺ B cells	29
T3 transitional B cells	CD38^dim CD10^low IgD⁺CD27⁻CD19⁺ B cells	29
T cells
T cells	CD3⁺T cells
Tc cells	CD3⁺CD8⁺ T cells
Th cells	CD3⁺CD4⁺ T cells
Naive Th cells	CD31⁻CD45RA⁺CD4⁺CD3⁺ T cells	30,31
Naive Tc cells	CD31⁻CD45RA⁺CD8⁺CD3⁺ T cells	30,31
Activated T cells	CD3⁺CD69⁺ T cells
CD31⁺ naive Th cells	CD31⁺CD45RA⁺CD4⁺CD3⁺ T cells	30,31
PD-1⁺ naive Tc cells	PD-1⁺CD45RA⁺ CD8⁺CD3⁺ T cells	32
Memory Th cells	PD-1-CD45RA⁻CD4⁺CD3⁺ T cells	32
Early Th	CD127⁺(IL-7Rα) CD25^int CD4⁺CD3⁺	33
Regulatory T cells
CD31⁺ naive T_reg cells	CD31⁺CD45RA⁺CD127^lowCD3⁺CD25⁺ T cells	34
Naive Treg cells	CD31⁻CD45RA⁺CD127^lowCD3⁺CD25⁺ T cells	34
PD-1⁺ memory T_reg	PD-1⁺CD45RA⁻CD127^lowCD3⁺CD25⁺ T cells	32
PD-1⁻ memory T_reg	PD-1⁻CD45RA⁻CD127^loCD3⁺CD25⁺ T cells	27
NK cells
CD56^dim NK cells	CD56^dim NK cells	8
Activated CD56^dim NK cells	CD56^dimCD69⁺ NK cells	8
Cytolytic CD56^bright NK cells	CD56^bright G^hi or CD335^hiCD56^brightP^hi	35-37
NK_regcells
NK_reg cells	CD56^brightCD335⁺	8
NK_reg noncytolytic	CD56^brightCD3⁻P^low or CD56^brightCD3⁻G^low	35-37

Cell population	Phenotype	References
B cells
CD19⁺ B cells	CD19⁺ lymphocytes	26-29
Nonswitched memory B cells	CD27⁺ IgD⁺ % of CD19⁺ B cells)	26
Naive B cells	CD38⁻CD10⁻ B cells (% of CD19⁺ B cells)	27
Mature naive B cells	IgD⁺CD27⁻CD19⁺	26
CD21^low B cells	CD19⁺CD21^low CD10^+/− / CD19⁺ B cells	28
Transitional B cells
T2 transitional B cells	CD38^intCD10^int IgD⁺CD27⁻ CD19⁺ B cells	29
T3 transitional B cells	CD38^dim CD10^low IgD⁺CD27⁻CD19⁺ B cells	29
T cells
T cells	CD3⁺T cells
Tc cells	CD3⁺CD8⁺ T cells
Th cells	CD3⁺CD4⁺ T cells
Naive Th cells	CD31⁻CD45RA⁺CD4⁺CD3⁺ T cells	30,31
Naive Tc cells	CD31⁻CD45RA⁺CD8⁺CD3⁺ T cells	30,31
Activated T cells	CD3⁺CD69⁺ T cells
CD31⁺ naive Th cells	CD31⁺CD45RA⁺CD4⁺CD3⁺ T cells	30,31
PD-1⁺ naive Tc cells	PD-1⁺CD45RA⁺ CD8⁺CD3⁺ T cells	32
Memory Th cells	PD-1-CD45RA⁻CD4⁺CD3⁺ T cells	32
Early Th	CD127⁺(IL-7Rα) CD25^int CD4⁺CD3⁺	33
Regulatory T cells
CD31⁺ naive T_reg cells	CD31⁺CD45RA⁺CD127^lowCD3⁺CD25⁺ T cells	34
Naive Treg cells	CD31⁻CD45RA⁺CD127^lowCD3⁺CD25⁺ T cells	34
PD-1⁺ memory T_reg	PD-1⁺CD45RA⁻CD127^lowCD3⁺CD25⁺ T cells	32
PD-1⁻ memory T_reg	PD-1⁻CD45RA⁻CD127^loCD3⁺CD25⁺ T cells	27
NK cells
CD56^dim NK cells	CD56^dim NK cells	8
Activated CD56^dim NK cells	CD56^dimCD69⁺ NK cells	8
Cytolytic CD56^bright NK cells	CD56^bright G^hi or CD335^hiCD56^brightP^hi	35-37
NK_regcells
NK_reg cells	CD56^brightCD335⁺	8
NK_reg noncytolytic	CD56^brightCD3⁻P^low or CD56^brightCD3⁻G^low	35-37

G, granzyme; P, perforin.

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