LM and specialized proresolving mediator profile in murine spleens
| Mediator . | Q1 . | Q3 . | AA normoxia . | SS normoxia . | AA hypoxia (3 hours) . | SS hypoxia (3 hours) . | AA hypoxia (18 hours) . | SS hypoxia (18 hours) . |
|---|---|---|---|---|---|---|---|---|
| DHA derived D-series Rv’s | ||||||||
| RvD1 | 375 | 215 | 182.6 ± 62.2 | 26.2 ± 7.0* | 372.1 ± 145.4 | 183.8 ± 62.7 | 414.7 ± 301.1 | 57.4 ± 12.9 |
| RvD2 | 375 | 141 | — | — | — | — | — | — |
| RvD3 | 375 | 181 | — | — | — | — | — | — |
| RvD4 | 375 | 101 | — | — | — | — | — | — |
| RvD5 | 359 | 199 | 17 ± 6.2 | 2.7 ± 1.6* | 48.9 ± 17.9 | 32.6 ± 13.5 | 81.9 ± 51.1 | 12 ± 4.5 |
| RvD6 | 359 | 101 | 62.1 ± 15.3 | 19.7 ± 3.4* | 139.4 ± 12.7 | 86.7 ± 15.5* | 201 ± 133.5 | 44.7 ± 9.7 |
| AT-RvD1 | 375 | 215 | 87.7 ± 35.2 | 8.6 ± 1.7* | 162 ± 44.1 | 126.5 ± 42.4 | 198.9 ± 163.1 | 38.1 ± 17.3 |
| AT-RvD3 | 375 | 181 | — | — | — | — | — | — |
| DHA-derived protectins and maresins | ||||||||
| PD1 | 359 | 153 | 2.8 ± 1.3 | 0.3 ± 0.0 | 4.2 ± 1.6 | 2.3 ± 1.0 | 0.9 ± 0.4 | 0.7 ± 0.3 |
| 10S,17S-diHDHA | 359 | 153 | 97.6 ± 32.5 | 18.6 ± 8.3* | 375.6 ± 127.5 | 159.5 ± 64.3 | 534 ± 278.1 | 57.4 ± 17.5 |
| 22-OH-PD1 | 375 | 153 | — | — | — | — | — | — |
| Maresin 1 | 359 | 221 | — | — | — | — | — | — |
| Maresin 2 | 359 | 250 | 54.3 ± 24.0 | 8.2 ± 3.6 | 254.9 ± 101.2 | 127.8 ± 49.0 | 580.2 ± 279.6 | 47.5 ± 9.6 |
| 7S,14S-diHDHA | 359 | 250 | — | — | — | — | — | — |
| 4S,14S-diHDHA | 359 | 101 | 24.7 ± 9.3 | 4.8 ± 2.3 | 139.4 ± 29.8 | 63.5 ± 24.6 | 297.2 ± 194.6 | 42.3 ± 18.4 |
| AA-derived LXs | ||||||||
| LXA4 | 351 | 115 | 45.3 ± 20.9 | 9.1 ± 3.5 | 65.3 ± 32.5 | 76.5 ± 43.5 | 56.3 ± 35.2 | 32.4 ± 15.1 |
| LXB4 | 351 | 221 | — | — | — | — | — | — |
| AA-derived proinflammatory mediators | ||||||||
| LTB4 | 335 | 195 | 770 ± 377.2 | 79.6 ± 27.6 | 1730.5 ± 493.8 | 1164.2 ± 467.2 | 4330.7 ± 2966.3 | 665.4 ± 305.2 |
| 20-OH-LTB4 | 351 | 195 | — | — | — | — | — | — |
| 20-COOH-LTB4 | 365 | 195 | — | — | — | — | — | — |
| 335 | 195 | 410.8 ± 190.5 | 35.7 ± 12.1 | 1101.4 ± 362.0 | 544.9 ± 219.0 | 2766 ± 1825.1 | 297.2 ± 128.3 | |
| LTC4 | 626 | 189 | 11 ± 3.9 | 7.9 ± 2.7 | 32.2 ± 16.1 | 19.9 ± 6.2 | 27.3 ± 6.3 | 18.7 ± 4.5 |
| LTD4 | 497 | 189 | 2.2 ± 1.0 | 0.8 ± 0.1 | 4.1 ± 1.9 | 2 ± 1.4 | 11.2 ± 6.4 | 3.3 ± 1.2 |
| LTE4 | 440 | 189 | 1.6 ± 0.3 | 0.7 ± 0.2* | 31.1 ± 26.9 | 11.1 ± 7.5 | 18.2 ± 9.9 | 3.4 ± 1.2 |
| Mediator . | Q1 . | Q3 . | AA normoxia . | SS normoxia . | AA hypoxia (3 hours) . | SS hypoxia (3 hours) . | AA hypoxia (18 hours) . | SS hypoxia (18 hours) . |
|---|---|---|---|---|---|---|---|---|
| DHA derived D-series Rv’s | ||||||||
| RvD1 | 375 | 215 | 182.6 ± 62.2 | 26.2 ± 7.0* | 372.1 ± 145.4 | 183.8 ± 62.7 | 414.7 ± 301.1 | 57.4 ± 12.9 |
| RvD2 | 375 | 141 | — | — | — | — | — | — |
| RvD3 | 375 | 181 | — | — | — | — | — | — |
| RvD4 | 375 | 101 | — | — | — | — | — | — |
| RvD5 | 359 | 199 | 17 ± 6.2 | 2.7 ± 1.6* | 48.9 ± 17.9 | 32.6 ± 13.5 | 81.9 ± 51.1 | 12 ± 4.5 |
| RvD6 | 359 | 101 | 62.1 ± 15.3 | 19.7 ± 3.4* | 139.4 ± 12.7 | 86.7 ± 15.5* | 201 ± 133.5 | 44.7 ± 9.7 |
| AT-RvD1 | 375 | 215 | 87.7 ± 35.2 | 8.6 ± 1.7* | 162 ± 44.1 | 126.5 ± 42.4 | 198.9 ± 163.1 | 38.1 ± 17.3 |
| AT-RvD3 | 375 | 181 | — | — | — | — | — | — |
| DHA-derived protectins and maresins | ||||||||
| PD1 | 359 | 153 | 2.8 ± 1.3 | 0.3 ± 0.0 | 4.2 ± 1.6 | 2.3 ± 1.0 | 0.9 ± 0.4 | 0.7 ± 0.3 |
| 10S,17S-diHDHA | 359 | 153 | 97.6 ± 32.5 | 18.6 ± 8.3* | 375.6 ± 127.5 | 159.5 ± 64.3 | 534 ± 278.1 | 57.4 ± 17.5 |
| 22-OH-PD1 | 375 | 153 | — | — | — | — | — | — |
| Maresin 1 | 359 | 221 | — | — | — | — | — | — |
| Maresin 2 | 359 | 250 | 54.3 ± 24.0 | 8.2 ± 3.6 | 254.9 ± 101.2 | 127.8 ± 49.0 | 580.2 ± 279.6 | 47.5 ± 9.6 |
| 7S,14S-diHDHA | 359 | 250 | — | — | — | — | — | — |
| 4S,14S-diHDHA | 359 | 101 | 24.7 ± 9.3 | 4.8 ± 2.3 | 139.4 ± 29.8 | 63.5 ± 24.6 | 297.2 ± 194.6 | 42.3 ± 18.4 |
| AA-derived LXs | ||||||||
| LXA4 | 351 | 115 | 45.3 ± 20.9 | 9.1 ± 3.5 | 65.3 ± 32.5 | 76.5 ± 43.5 | 56.3 ± 35.2 | 32.4 ± 15.1 |
| LXB4 | 351 | 221 | — | — | — | — | — | — |
| AA-derived proinflammatory mediators | ||||||||
| LTB4 | 335 | 195 | 770 ± 377.2 | 79.6 ± 27.6 | 1730.5 ± 493.8 | 1164.2 ± 467.2 | 4330.7 ± 2966.3 | 665.4 ± 305.2 |
| 20-OH-LTB4 | 351 | 195 | — | — | — | — | — | — |
| 20-COOH-LTB4 | 365 | 195 | — | — | — | — | — | — |
| 335 | 195 | 410.8 ± 190.5 | 35.7 ± 12.1 | 1101.4 ± 362.0 | 544.9 ± 219.0 | 2766 ± 1825.1 | 297.2 ± 128.3 | |
| LTC4 | 626 | 189 | 11 ± 3.9 | 7.9 ± 2.7 | 32.2 ± 16.1 | 19.9 ± 6.2 | 27.3 ± 6.3 | 18.7 ± 4.5 |
| LTD4 | 497 | 189 | 2.2 ± 1.0 | 0.8 ± 0.1 | 4.1 ± 1.9 | 2 ± 1.4 | 11.2 ± 6.4 | 3.3 ± 1.2 |
| LTE4 | 440 | 189 | 1.6 ± 0.3 | 0.7 ± 0.2* | 31.1 ± 26.9 | 11.1 ± 7.5 | 18.2 ± 9.9 | 3.4 ± 1.2 |
See supplemental Methods for extractions and LM profiling via LC-MS-MS. Data are expressed as picogram per 150 mg of tissue (average ± SEM).
Q, quadrupole.
—, below the limit of detection (limit of detection ∼0.1 pg).
P < .05, SS normoxia vs AA normoxia or SS hypoxia vs AA hypoxia, 1-tailed t test.