LM and specialized proresolving mediator profile in murine spleens
| Mediator . | Q1 . | Q3 . | AA normoxia . | SS normoxia . | AA Hypoxia 3 h . | SS Hypoxia 3 h . | AA Hypoxia 18 h . | SS Hypoxia 18 h . |
|---|---|---|---|---|---|---|---|---|
| EPA derived E-series Rv’s | ||||||||
| RvE1 | 349 | 195 | — | — | — | — | — | — |
| RvE2 | 333 | 199 | — | — | — | — | — | — |
| RvE3 | 333 | 201 | — | — | — | — | — | — |
| AA-derived LXs | ||||||||
| 5S,15S-diHETE | 335 | 115 | — | — | — | — | — | — |
| AT-LXA4 | 351 | 115 | — | — | — | — | — | — |
| AT-LXB4 | 351 | 221 | — | — | — | — | — | — |
| AA-derived prostaglandins and thromboxane | ||||||||
| PGD2 | 351 | 233 | 106 818.4 ± 45 244.9 | 15 806.4 ± 5 317.5 | 143 978.5 ± 63 799.0 | 110 797.8 ± 60 169.4 | 11 5139 ± 86 296.2 | 23 629.5 ± 8 314.5 |
| PGE2 | 351 | 189 | 11 615.8 ± 6 061.4 | 1 097.3 ± 513.4 | 37 585.9 ± 22 433.2 | 12 464.7 ± 8 645.4 | 25 222.2 ± 16 477.6 | 3 309 ± 1 284.3 |
| PGF2α | 353 | 193 | 23 853.8 ± 10 106.7 | 6 806.9 ± 2 947.7 | 39 083.1 ± 14 750.2 | 69 788.8 ± 43 692.4 | 44 996.9 ± 31 680.5 | 14 777.4 ± 5 575.2 |
| TXB2 | 369 | 169 | 83 722 ± 40 545.4 | 12 727.9 ± 5 177.0 | 125 070 ± 61 312.8 | 123 633.2 ± 71 400.0 | 113 006.4 ± 80 794.7 | 28 989.6 ± 11 183.7 |
| Pathway markers and fatty acid precursors | ||||||||
| 17-HDHA | 343 | 245 | 2 429.2 ± 1 031.0 | 473 ± 129.6 | 5 421.5 ± 1 098.9 | 3 147.3 ± 1 119.2 | 6 997.1 ± 4 894.8 | 918.8 ± 243.4 |
| 14-HDHA | 343 | 205 | 8 065.8 ± 4 548.3 | 1 158.5 ± 331.9 | 17 791.1 ± 3 854.2 | 6 879.6 ± 3 009.2* | 31 442 ± 21 197.7 | 4 024.8 ± 1 742.5 |
| 7-HDHA | 343 | 141 | 96.5 ± 40.3 | 13.7 ± 2.8 | 184.7 ± 68.7 | 140.2 ± 53.3 | 230.5 ± 197.4 | 29.5 ± 11.8 |
| 4-HDHA | 343 | 101 | 140.4 ± 42.2 | 45.8 ± 1.8* | 444.1 ± 114.1 | 288.6 ± 74.5 | 508.3 ± 370.2 | 136.8 ± 74.5 |
| DHA | 327 | 283 | 50 229.9 ± 19 687.8 | 13 583.4 ± 5 231.5 | 72 918.8 ± 24 137.3 | 47 613.8 ± 25 060.1 | 58 042.8 ± 48 021.2 | 12 676.2 ± 4 101.8 |
| 18-HEPE | 317 | 259 | 157.4 ± 46.3 | 33.8 ± 12.6* | 289.4 ± 112.6 | 320.4 ± 104.4 | 691.4 ± 542.2 | 138.7 ± 51.4 |
| 15-HEPE | 317 | 219 | 307.6 ± 95.6 | 55.3 ± 8.4* | 822.4 ± 288.2 | 447.6 ± 150.2 | 1 260.6 ± 750.7 | 192.4 ± 54.6 |
| 12-HEPE | 317 | 179 | 3 248.4 ± 1 964.4 | 293.6 ± 142.2 | 5 151.1 ± 2 327.3 | 2 112.2 ± 1 434.3 | 20 410.5 ± 11 056.4 | 4 921.5 ± 2 433.9 |
| 5-HEPE | 317 | 115 | 42.9 ± 13.1 | 13.3 ± 4.3 | 79.6 ± 22.7 | 51.4 ± 18.5 | 171.8 ± 136.4 | 46.6 ± 19.2 |
| EPA | 301 | 257 | 10 602.8 ± 3 091.4 | 4 369.5 ± 1 067.0 | 11 041.8 ± 1 016.8 | 13 003.7 ± 5 243.7 | 17 125.2 ± 12 938.0 | 6 686.4 ± 2 168.7 |
| 15-HETE | 319 | 219 | 11 194.4 ± 4 254.7 | 1 980.9 ± 496.7 | 19 258.9 ± 2 412.7 | 10 224.9 ± 3 767.5 | 21 184 ± 15 314.6 | 3 729.2 ± 957.0 |
| 12-HETE | 319 | 179 | 33 634 ± 17 891.3 | 4 636.8 ± 1 470.8 | 48 734.2 ± 15 019.0 | 21 222.1 ± 8 947.6 | 57 858.1 ± 43 233.4 | 10 973.5 ± 4 187.1 |
| 5-HETE | 319 | 115 | 943.2 ± 298.1 | 266.5 ± 28.0* | 2 193.2 ± 456.2 | 1 674.6 ± 540.6 | 2 646.6 ± 1 964.8 | 566.7 ± 228.1 |
| AA | 303 | 257 | 89 921 ± 36 814.6 | 21 131.3 ± 8 190.9 | 125 923.7 ± 45 063.1 | 84 438.2 ± 47 853.7 | 92 614.7 ± 76 142.5 | 18 565.3 ± 5 792.9 |
| Mediator . | Q1 . | Q3 . | AA normoxia . | SS normoxia . | AA Hypoxia 3 h . | SS Hypoxia 3 h . | AA Hypoxia 18 h . | SS Hypoxia 18 h . |
|---|---|---|---|---|---|---|---|---|
| EPA derived E-series Rv’s | ||||||||
| RvE1 | 349 | 195 | — | — | — | — | — | — |
| RvE2 | 333 | 199 | — | — | — | — | — | — |
| RvE3 | 333 | 201 | — | — | — | — | — | — |
| AA-derived LXs | ||||||||
| 5S,15S-diHETE | 335 | 115 | — | — | — | — | — | — |
| AT-LXA4 | 351 | 115 | — | — | — | — | — | — |
| AT-LXB4 | 351 | 221 | — | — | — | — | — | — |
| AA-derived prostaglandins and thromboxane | ||||||||
| PGD2 | 351 | 233 | 106 818.4 ± 45 244.9 | 15 806.4 ± 5 317.5 | 143 978.5 ± 63 799.0 | 110 797.8 ± 60 169.4 | 11 5139 ± 86 296.2 | 23 629.5 ± 8 314.5 |
| PGE2 | 351 | 189 | 11 615.8 ± 6 061.4 | 1 097.3 ± 513.4 | 37 585.9 ± 22 433.2 | 12 464.7 ± 8 645.4 | 25 222.2 ± 16 477.6 | 3 309 ± 1 284.3 |
| PGF2α | 353 | 193 | 23 853.8 ± 10 106.7 | 6 806.9 ± 2 947.7 | 39 083.1 ± 14 750.2 | 69 788.8 ± 43 692.4 | 44 996.9 ± 31 680.5 | 14 777.4 ± 5 575.2 |
| TXB2 | 369 | 169 | 83 722 ± 40 545.4 | 12 727.9 ± 5 177.0 | 125 070 ± 61 312.8 | 123 633.2 ± 71 400.0 | 113 006.4 ± 80 794.7 | 28 989.6 ± 11 183.7 |
| Pathway markers and fatty acid precursors | ||||||||
| 17-HDHA | 343 | 245 | 2 429.2 ± 1 031.0 | 473 ± 129.6 | 5 421.5 ± 1 098.9 | 3 147.3 ± 1 119.2 | 6 997.1 ± 4 894.8 | 918.8 ± 243.4 |
| 14-HDHA | 343 | 205 | 8 065.8 ± 4 548.3 | 1 158.5 ± 331.9 | 17 791.1 ± 3 854.2 | 6 879.6 ± 3 009.2* | 31 442 ± 21 197.7 | 4 024.8 ± 1 742.5 |
| 7-HDHA | 343 | 141 | 96.5 ± 40.3 | 13.7 ± 2.8 | 184.7 ± 68.7 | 140.2 ± 53.3 | 230.5 ± 197.4 | 29.5 ± 11.8 |
| 4-HDHA | 343 | 101 | 140.4 ± 42.2 | 45.8 ± 1.8* | 444.1 ± 114.1 | 288.6 ± 74.5 | 508.3 ± 370.2 | 136.8 ± 74.5 |
| DHA | 327 | 283 | 50 229.9 ± 19 687.8 | 13 583.4 ± 5 231.5 | 72 918.8 ± 24 137.3 | 47 613.8 ± 25 060.1 | 58 042.8 ± 48 021.2 | 12 676.2 ± 4 101.8 |
| 18-HEPE | 317 | 259 | 157.4 ± 46.3 | 33.8 ± 12.6* | 289.4 ± 112.6 | 320.4 ± 104.4 | 691.4 ± 542.2 | 138.7 ± 51.4 |
| 15-HEPE | 317 | 219 | 307.6 ± 95.6 | 55.3 ± 8.4* | 822.4 ± 288.2 | 447.6 ± 150.2 | 1 260.6 ± 750.7 | 192.4 ± 54.6 |
| 12-HEPE | 317 | 179 | 3 248.4 ± 1 964.4 | 293.6 ± 142.2 | 5 151.1 ± 2 327.3 | 2 112.2 ± 1 434.3 | 20 410.5 ± 11 056.4 | 4 921.5 ± 2 433.9 |
| 5-HEPE | 317 | 115 | 42.9 ± 13.1 | 13.3 ± 4.3 | 79.6 ± 22.7 | 51.4 ± 18.5 | 171.8 ± 136.4 | 46.6 ± 19.2 |
| EPA | 301 | 257 | 10 602.8 ± 3 091.4 | 4 369.5 ± 1 067.0 | 11 041.8 ± 1 016.8 | 13 003.7 ± 5 243.7 | 17 125.2 ± 12 938.0 | 6 686.4 ± 2 168.7 |
| 15-HETE | 319 | 219 | 11 194.4 ± 4 254.7 | 1 980.9 ± 496.7 | 19 258.9 ± 2 412.7 | 10 224.9 ± 3 767.5 | 21 184 ± 15 314.6 | 3 729.2 ± 957.0 |
| 12-HETE | 319 | 179 | 33 634 ± 17 891.3 | 4 636.8 ± 1 470.8 | 48 734.2 ± 15 019.0 | 21 222.1 ± 8 947.6 | 57 858.1 ± 43 233.4 | 10 973.5 ± 4 187.1 |
| 5-HETE | 319 | 115 | 943.2 ± 298.1 | 266.5 ± 28.0* | 2 193.2 ± 456.2 | 1 674.6 ± 540.6 | 2 646.6 ± 1 964.8 | 566.7 ± 228.1 |
| AA | 303 | 257 | 89 921 ± 36 814.6 | 21 131.3 ± 8 190.9 | 125 923.7 ± 45 063.1 | 84 438.2 ± 47 853.7 | 92 614.7 ± 76 142.5 | 18 565.3 ± 5 792.9 |
See supplemental Methods for extractions and LM profiling via LC-MS-MS. Results are expressed as picogram per 150 mg of tissue (average ± standard error of the mean).
Q, quadrupole.
—, below the limit of detection (limit of detection ∼0.1 pg).
P < 0.05, SS normoxia vs AA normoxia or SS hypoxia vs AA hypoxia, 1-tailed t-test.