Fc glycosylation (relative expression levels) of total IgG1 vs specific IgG1 of TRALI donor anti-HLA antibodies vs control donor anti-HLA antibodies
| Fc glycosylation . | Total IgG1 . | Specific IgG1 . | Total IgG1 vs Specific IgG1, P . |
|---|---|---|---|
| TRALI donor anti-HLA antibodies | |||
| Fucosylation | 0.93 ± 0.03 | 0.85 ± 0.05 | P = .0002, *** |
| Galactosylation | 0.61 ± 0.05 | 0.60 ± 0.09 | NS |
| Sialylation | 0.17 ± 0.03 | 0.16 ± 0.05 | NS |
| Bisecting GlcNAc | 0.15 ± 0.03 | 0.20 ± 0.03 | P = .0005, *** |
| Control donor anti-HLA antibodies | |||
| Fucosylation | 0.94 ± 0.03 | 0.86 ± 0.09 | P = .0008, *** |
| Galactosylation | 0.57 ± 0.10 | 0.62 ± 0.05 | P = .0062, ** |
| Sialylation | 0.15 ± 0.05 | 0.15 ± 0.02 | NS |
| Bisecting GlcNAc | 0.15 ± 0.03 | 0.15 ± 0.02 | NS |
| Fc glycosylation . | Total IgG1 . | Specific IgG1 . | Total IgG1 vs Specific IgG1, P . |
|---|---|---|---|
| TRALI donor anti-HLA antibodies | |||
| Fucosylation | 0.93 ± 0.03 | 0.85 ± 0.05 | P = .0002, *** |
| Galactosylation | 0.61 ± 0.05 | 0.60 ± 0.09 | NS |
| Sialylation | 0.17 ± 0.03 | 0.16 ± 0.05 | NS |
| Bisecting GlcNAc | 0.15 ± 0.03 | 0.20 ± 0.03 | P = .0005, *** |
| Control donor anti-HLA antibodies | |||
| Fucosylation | 0.94 ± 0.03 | 0.86 ± 0.09 | P = .0008, *** |
| Galactosylation | 0.57 ± 0.10 | 0.62 ± 0.05 | P = .0062, ** |
| Sialylation | 0.15 ± 0.05 | 0.15 ± 0.02 | NS |
| Bisecting GlcNAc | 0.15 ± 0.03 | 0.15 ± 0.02 | NS |
Statistical analysis: 2-tailed paired Student t test. **P < .01, ***P < .001.