CB cell therapy characteristics and effect on chimerism and response
| . | All patients (N = 31) . | Chimerism . | P . | Response . | P . | ||
|---|---|---|---|---|---|---|---|
| Yes (n = 12) . | No (n = 19) . | Yes (n = 13) . | No (n = 18) . | ||||
| Precryopreservation TNC, ×107/kg | .289 | .645 | |||||
| Median | 1.40 | 1.45 | 1.40 | 1.40 | 1.40 | ||
| Range | 0.70-2.40 | 1.10-2.40 | 0.70-2.30 | 0.70-2.40 | 1.00-2.30 | ||
| Precryopreservation CD34, ×103/kg | .172 | .523 | |||||
| Median | 34.00 | 47.00 | 32.00 | 38.00 | 32.50 | ||
| Range | 16.00-101.00 | 16.00-101.00 | 19.00-87.00 | 16.00-101.00 | 19.00-87.00 | ||
| Precryopreservation total lymphocytes,×106 | .011 | .464 | |||||
| Median | 321.07 | 429.25 | 269.68 | 395.18 | 299.38 | ||
| Range | 124.72-520.00 | 184.87-510.63 | 124.72-520 | 159.10-510.60 | 124.72-520.00 | ||
| Precryopreservation total lymphocytes,×106/kg | .001 | .228 | |||||
| Median | 5.26 | 6.12 | 4.11 | 5.43 | 4.85 | ||
| Range | 1.40-10.21 | 3.08-10.21 | 1.40-6.83 | 2.04-10.21 | 1.40-7.11 | ||
| Lymphocytes preprocessing, % | .032 | .541 | |||||
| Median | 32.75 | 36.90 | 31.70 | 33.05 | 32.20 | ||
| Range | 18.00-47.80 | 24.20-47.80 | 18.00-46.20 | 18.00-47.80 | 20.90-46.20 | ||
| Lymphocytes precryopreservation, % | .003 | .199 | |||||
| Median | 37.20 | 42.20 | 33.90 | 41.10 | 34.10 | ||
| Range | 13.60-63.20 | 30.90-63.20 | 13.60-48.60 | 16.50-50.00 | 13.60-63.20 | ||
| Lymphocytes postthaw, % | .016 | .075 | |||||
| Median | 45.00 | 48.00 | 38.00 | 54.00 | 39.50 | ||
| Range | 24.00-70.00 | 40.60-70.00 | 24.00-57.00 | 46.00-72.00 | 24.00-70.00 | ||
| Postthaw CD3 cells,×106/kg | .017 | .260 | |||||
| Median | 3.50 | 3.70 | 2.90 | 3.50 | 3.45 | ||
| Range | 1.30-6.60 | 2.60-6.60 | 1.30-6.20 | 1.60-6.60 | 1.30-6.20 | ||
| Postthaw CD34 cells,×103/kg | .14 | .532 | |||||
| Median | 30.00 | 43.00 | 29.00 | 30.00 | 30.00 | ||
| Range | 10.00-99.00 | 13.00-90.00 | 10.00-70.00 | 10.00-90.00 | 16.00-70.00 | ||
| HLA match with patient, n* | .081 | .047 | |||||
| ≥5/6 | 20 | 10 | 10 | 11 | 9 | ||
| <5/6 | 11 | 2 | 9 | 2 | 9 | ||
| HLA allele level match with patient, n* | .128 | .739 | |||||
| ≥6/8 | 18 | 9 | 9 | 8 | 10 | ||
| <6/8 | 13 | 3 | 10 | 5 | 8 | ||
| NIMA match with patient, n | .355 | .767 | |||||
| Yes | 8 | 2 | 6 | 3 | 5 | ||
| No | 23 | 10 | 13 | 10 | 13 | ||
| Sex match with patient, n | .440 | .284 | |||||
| Yes | 13 | 4 | 9 | 4 | 9 | ||
| No | 18 | 8 | 10 | 9 | 9 | ||
| CBU storage duration, y | .834 | .733 | |||||
| Median | 11 | 11.47 | 11.29 | 11.07 | 11.95 | ||
| Range | 6-18 | 7.98-18.13 | 5.69-16.05 | 7.38-18.13 | 5.60-16.05 | ||
| . | All patients (N = 31) . | Chimerism . | P . | Response . | P . | ||
|---|---|---|---|---|---|---|---|
| Yes (n = 12) . | No (n = 19) . | Yes (n = 13) . | No (n = 18) . | ||||
| Precryopreservation TNC, ×107/kg | .289 | .645 | |||||
| Median | 1.40 | 1.45 | 1.40 | 1.40 | 1.40 | ||
| Range | 0.70-2.40 | 1.10-2.40 | 0.70-2.30 | 0.70-2.40 | 1.00-2.30 | ||
| Precryopreservation CD34, ×103/kg | .172 | .523 | |||||
| Median | 34.00 | 47.00 | 32.00 | 38.00 | 32.50 | ||
| Range | 16.00-101.00 | 16.00-101.00 | 19.00-87.00 | 16.00-101.00 | 19.00-87.00 | ||
| Precryopreservation total lymphocytes,×106 | .011 | .464 | |||||
| Median | 321.07 | 429.25 | 269.68 | 395.18 | 299.38 | ||
| Range | 124.72-520.00 | 184.87-510.63 | 124.72-520 | 159.10-510.60 | 124.72-520.00 | ||
| Precryopreservation total lymphocytes,×106/kg | .001 | .228 | |||||
| Median | 5.26 | 6.12 | 4.11 | 5.43 | 4.85 | ||
| Range | 1.40-10.21 | 3.08-10.21 | 1.40-6.83 | 2.04-10.21 | 1.40-7.11 | ||
| Lymphocytes preprocessing, % | .032 | .541 | |||||
| Median | 32.75 | 36.90 | 31.70 | 33.05 | 32.20 | ||
| Range | 18.00-47.80 | 24.20-47.80 | 18.00-46.20 | 18.00-47.80 | 20.90-46.20 | ||
| Lymphocytes precryopreservation, % | .003 | .199 | |||||
| Median | 37.20 | 42.20 | 33.90 | 41.10 | 34.10 | ||
| Range | 13.60-63.20 | 30.90-63.20 | 13.60-48.60 | 16.50-50.00 | 13.60-63.20 | ||
| Lymphocytes postthaw, % | .016 | .075 | |||||
| Median | 45.00 | 48.00 | 38.00 | 54.00 | 39.50 | ||
| Range | 24.00-70.00 | 40.60-70.00 | 24.00-57.00 | 46.00-72.00 | 24.00-70.00 | ||
| Postthaw CD3 cells,×106/kg | .017 | .260 | |||||
| Median | 3.50 | 3.70 | 2.90 | 3.50 | 3.45 | ||
| Range | 1.30-6.60 | 2.60-6.60 | 1.30-6.20 | 1.60-6.60 | 1.30-6.20 | ||
| Postthaw CD34 cells,×103/kg | .14 | .532 | |||||
| Median | 30.00 | 43.00 | 29.00 | 30.00 | 30.00 | ||
| Range | 10.00-99.00 | 13.00-90.00 | 10.00-70.00 | 10.00-90.00 | 16.00-70.00 | ||
| HLA match with patient, n* | .081 | .047 | |||||
| ≥5/6 | 20 | 10 | 10 | 11 | 9 | ||
| <5/6 | 11 | 2 | 9 | 2 | 9 | ||
| HLA allele level match with patient, n* | .128 | .739 | |||||
| ≥6/8 | 18 | 9 | 9 | 8 | 10 | ||
| <6/8 | 13 | 3 | 10 | 5 | 8 | ||
| NIMA match with patient, n | .355 | .767 | |||||
| Yes | 8 | 2 | 6 | 3 | 5 | ||
| No | 23 | 10 | 13 | 10 | 13 | ||
| Sex match with patient, n | .440 | .284 | |||||
| Yes | 13 | 4 | 9 | 4 | 9 | ||
| No | 18 | 8 | 10 | 9 | 9 | ||
| CBU storage duration, y | .834 | .733 | |||||
| Median | 11 | 11.47 | 11.29 | 11.07 | 11.95 | ||
| Range | 6-18 | 7.98-18.13 | 5.69-16.05 | 7.38-18.13 | 5.60-16.05 | ||
P values in bold are <.05.
CBU with the highest HLA match were preferred during the final selection.