TCRγ locus is rearranged and TCRδ locus is deleted in ILC2s. (A-C) Genomic PCR of select TCRγ, TCRδ, and TCRβ gene rearrangement permutations in lung ILC2s. (Α-Β) Splenic γδT cells and lung γδT cells were used as positive controls and (A-C) lung ILC2s from Rag1^−/− mice were used as a negative control. Nonspecific (n.s.) amplicon in panel C is marked by an arrow. Gapdh was used as a control for genomic PCR. (D) Copy number variation assay of WT lung ILC2s and γδT cells by real-time qPCR. Splenocytes from Rag1^−/− mice were used as a reference since they retain 2 copies of germline TCRδ alleles. Significance was determined by Student t test. **P < .01; ***P < .001. n.s., not significant (panel D).