Frequent nonproductive TCR locus rearrangements in the ILC2 lineage. Bulk next-generation sequencing of rearranged genomic Vγ2-Jγ1 and Vγ1.2-Jγ2 amplicons of lung ILC2s and γδT cells isolated from WT mice. Data were collected from 2 independent sequencing runs. (A) tcR pipeline was used to compare TCRγ clonotype diversity between ILC2s and γδT cells. (B) Relative abundance of in-frame or out-of-frame TCRγ gene rearrangement of top 20 most frequent clonotypes was calculated using MiXCR and vdjtools. (C) In-depth CDR3 sequence analysis of top 20 Vγ2 repertoire. Representative ILC2 and γδT samples were chosen to portray the characteristics of nonfunctional and functional Vγ2-Jγ1. Nonfunctional CDR3 amino acid sequences (CDR3aa) are highlighted in red. (*) Demarcates premature stop codons and (_) denotes frameshifts caused by indels.