Evaluation of ILC2 frequency in models that are incapable of generating functional T cells. (A) Thymus, spleen, and lung tissues were isolated and put into single-cell suspensions from adult (7 to 10 weeks old) WT, Rag1^−/− and Tcrδ/β^−/− mice. ILC2 frequency and absolute number were measured using the gating strategy shown in the representative FACS plots. ILC2s were characterized based on CD45.2⁺Lin^–Thy1.2^hiCD25⁺CD127⁺ surface markers, and the data were collected on a Beckman Coulter Cytoflex flow cytometer. (B) Percent frequencies of ILC2s in total CD45.2⁺ cells and their total numbers in the thymus, spleen, and lung. Significance was determined by Student t test (n > 4). *P < .05; **P < .005; ***P < .0005; P < .0001 is not labeled. Representative data from 2 independent experiments are shown.