Figure 4.
Il18tg mice have enhanced CD8 T-cell activation and IFNg production but gene expression similar to WT mice. Mice of the indicated genotypes were infected with LCMV-Arm and assessed for (A) absolute number of splenic CD8+ T lymphocytes. On day 8 of infection, mice were further evaluated for (B) the percentage of splenic CD8+ T lymphocytes expressing KLRG1+, (C) cytokine expression after stimulation with GP33 peptide, and the percentage of effector CD8 T lymphocytes expressing inhibitory markers. *Adjusted P < .05, **P < .01, ***P < .001, ***P < .0001 by 1-way ANOVA with Tukey post-test. Significance is only shown for comparisons where adjusted P < .05. Error bars represent SEM. Results are representative of at least 2 independent experiments with a minimum of 3 mice per genotype. (D) RNASeq analysis of GP33-antigen specific CD8 T lymphocytes including (i) relative expression of selected genes (normalized to the average expression of WT), (ii) Venn diagram of differentially expressed genes, and (iii) Gene Set Enrichment Analysis (GSEA) of the Prf1−/− vs Il18tg comparison showing the most highly enriched MSigDb C7 (immunologic signature) CD8 T-cell upregulation gene set (from GSE9650). See supplemental Data for complete differential expression and GSEA data. FDR, false discovery rate; NES, normalized enrichment score.

Il18tg mice have enhanced CD8 T-cell activation and IFNg production but gene expression similar to WT mice. Mice of the indicated genotypes were infected with LCMV-Arm and assessed for (A) absolute number of splenic CD8+ T lymphocytes. On day 8 of infection, mice were further evaluated for (B) the percentage of splenic CD8+ T lymphocytes expressing KLRG1+, (C) cytokine expression after stimulation with GP33 peptide, and the percentage of effector CD8 T lymphocytes expressing inhibitory markers. *Adjusted P < .05, **P < .01, ***P < .001, ***P < .0001 by 1-way ANOVA with Tukey post-test. Significance is only shown for comparisons where adjusted P < .05. Error bars represent SEM. Results are representative of at least 2 independent experiments with a minimum of 3 mice per genotype. (D) RNASeq analysis of GP33-antigen specific CD8 T lymphocytes including (i) relative expression of selected genes (normalized to the average expression of WT), (ii) Venn diagram of differentially expressed genes, and (iii) Gene Set Enrichment Analysis (GSEA) of the Prf1−/− vs Il18tg comparison showing the most highly enriched MSigDb C7 (immunologic signature) CD8 T-cell upregulation gene set (from GSE9650). See supplemental Data for complete differential expression and GSEA data. FDR, false discovery rate; NES, normalized enrichment score.

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