19-CAR-ML NK cells effectively control lymphoma targets in vivo in NSG mice. (A) Schema of in vivo studies using luciferase (luc)-expressing Raji. Irradiated mice received 1 × 10⁵ Raji-luc cells IV followed by 1.9 × 10⁵ 33-CAR-ML or 19-CAR-ML NK cells (total, 3 × 10⁶ to 5 × 10⁶ IV on day 0). Lymphoma burden was determined by every-other-day BLI. (B) Representative BLI images from mice at indicated time points. (C) Summary BLI data of tumor burden of each group on day 24. Day 24 data are presented, because this was the longest time point that included all the mice in the untreated group before mortality from lymphoma. Data shown as bar graph with mean ± SEM (n = 7-11 total mice from 2 independent experiments). (D) Summary of BLI data of the tumor burden of each group monitored for 28 days after cell infusion. Mean ± standard deviation shown (n = 10-11; 2 independent experiments) (E) Kaplan-Meier survival curve of mice receiving tumor only (blue line), 33-CAR-ML NK cells (red line), or 19-CAR-ML NK cells (green line). Data were compared with a linear mixed model followed by Student t test for 19-CAR-ML vs 33-CAR-ML NK cells (D) and log-rank test (E). *P < .05, **P < .01, ***P < .001, ****P < .0001.