CD200⁺LSCs can be prospectively separated from HCSs. (A) Flow cytometry plots showing gating strategy used to separate CD200^– and CD200⁺ blasts (CD200 Blast Sort) and to separate CD200⁺ LSCs from CD200⁺ HSCs (Expanded CD200 Sort). (B) Human CD45⁺ engraftment in injected femur following transplantation of sorted populations at indicated doses from samples AML2 and AML7. Each symbol represents 1 mouse; red symbols indicate leukemic grafts, green symbols indicate multilineage grafts, black symbols indicate no engraftment; mean ± standard deviation is shown for each cohort of mice.