American Society of Hematology

Figure 4.

$CD200 captures both CD34–and CD34+LSCs. (A) Flow cytometric analysis of CD34 and CD38 expression on CD200+ and CD200− blasts from 7 AML samples with CD200+ LSCs. Engrafting fractions containing LSCs as determined by xenotransplantation assays are indicated by green boxes. (B) Sorting gates and level of human CD45+ engraftment in injected femur following transplantation at indicated doses of sorted populations from samples AML5, AML21 and AML63. (C) Sorting gates and level of human CD45+ engraftment in injected femur following transplantation at indicated doses of sorted populations from sample AML5. For panels B and C, each symbol represents 1 mouse; red symbols indicate leukemic grafts, black symbols indicate no engraftment; mean ± standard deviation is shown for each cohort of mice.$

CD200 captures both CD34^–and CD34⁺LSCs. (A) Flow cytometric analysis of CD34 and CD38 expression on CD200⁺ and CD200⁻ blasts from 7 AML samples with CD200⁺ LSCs. Engrafting fractions containing LSCs as determined by xenotransplantation assays are indicated by green boxes. (B) Sorting gates and level of human CD45⁺ engraftment in injected femur following transplantation at indicated doses of sorted populations from samples AML5, AML21 and AML63. (C) Sorting gates and level of human CD45⁺ engraftment in injected femur following transplantation at indicated doses of sorted populations from sample AML5. For panels B and C, each symbol represents 1 mouse; red symbols indicate leukemic grafts, black symbols indicate no engraftment; mean ± standard deviation is shown for each cohort of mice.

This Feature Is Available To Subscribers Only