Detection of progenitor programs in CD200⁺LSCs from AMLs with NPM1 mutations. (A) GSEA of gene expression profiles of CD200⁺ LSC-containing and CD200⁻ non-LSC-containing blasts from 8 AML samples, using primitive HSC/progenitor-like and mature myeloid-like gene signatures. (B) Single sample GSEA of gene expression profiles of bulk cells from 4 NPM1-mutated AML samples (AML3, AML7, AML13, and AML21) and LSC-enriched CD200⁺ blasts from 6 NPM1-mutated AML cases (AML2, AML3, AML5, AML7, AML13, and AML21), using HSC/progenitor-like and myeloid-like gene signatures. *P < .05. FDR, false discovery rate; NES, normalized enrichment score.