Figure 2.
Allele-specific methylation of ASNS in BCP-ALL cell lines. (A) ASNS methylation in BCP-ALL cell lines. The top panel shows a heat map of 10 representative cell lines. The bottom panel indicates distribution of the mean percent methylation in 83 BCP-ALL cell lines. (B) Typical histograms of the mean percent methylation in weakly, intermediately, and highly methylated cell lines. Vertical axes indicate the frequency of NGS reads, and the horizontal axes indicate the level of methylation. (C) PCR using genomic DNA after incubation with or without Aor13HI as a template in intermediately methylated cell lines with a heterozygous genotype of a tandem repeat polymorphism. The bottom panel shows histograms of ASNS methylation. (D) Sequences of 3 single nucleotide polymorphisms (SNPs) located in intron 2 of the ASNS gene in weakly and highly methylated cell lines.

Allele-specific methylation of ASNS in BCP-ALL cell lines. (A) ASNS methylation in BCP-ALL cell lines. The top panel shows a heat map of 10 representative cell lines. The bottom panel indicates distribution of the mean percent methylation in 83 BCP-ALL cell lines. (B) Typical histograms of the mean percent methylation in weakly, intermediately, and highly methylated cell lines. Vertical axes indicate the frequency of NGS reads, and the horizontal axes indicate the level of methylation. (C) PCR using genomic DNA after incubation with or without Aor13HI as a template in intermediately methylated cell lines with a heterozygous genotype of a tandem repeat polymorphism. The bottom panel shows histograms of ASNS methylation. (D) Sequences of 3 single nucleotide polymorphisms (SNPs) located in intron 2 of the ASNS gene in weakly and highly methylated cell lines.

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