Association between L-asparaginase sensitivity and ASNS methylation in primary BCP-ALL samples. (A-B) Association between percent cell viabilities at 0.01 IU/mL of L-asparaginase and ASNS methylation in BCP-ALL samples at (A) diagnosis and at (B) relapse. Red circles, yellow rhomboids, and blue squares demonstrate highly, intermediately, and weakly methylated samples, respectively. P values in a Mann-Whitney U test are indicated. (C) ASNS methylation in BCP-ALL samples. The left panel indicates ASNS methylation in the samples at diagnosis (left) and relapse (right). The P value in a Mann-Whitney U test is indicated. Solid lines indicate paired samples at diagnosis and relapse of 4 patients. In the right panel, histograms indicate ASNS methylation in 4 paired samples. Boxes indicate mean cell viability with 0.01 IU/mL of L-asparaginase in a triplicated assay with standard deviation. Arrows indicate months before relapse. Correlation coefficients and P values are shown at the top of the panels.