Favorable metabolic reprogramming of CD38^KO NK cells. (A) Heat map of DEGs of significantly altered pathways (cholesterol biosynthesis and OXPHOS) as determined by IPA, based on normalized RNA-seq data of paired CD38^WT and CD38^KO NK cells (n = 6). (B) Principle components analysis (PCA) of DEGs, showing consistent effect of CD38 deletion for each donor despite wide interdonor variability. (C) Summarized data of metabolic analysis of paired CD38^WT and CD38^KO NK cells (n = 3; mean ± SD). (D) Graphical analysis of basal OCR, ECAR, OCR/ECAR, and spare respiratory capacity (SRC) derived from (C). All experiments were achieved using quintuplicate samples. FCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone; ROT/AA, rotenone and antimycin A.