Western blot for A1AT in liver lysates. (A) Liver lysates prepared from Lman1^cgt/cgt, Lman1^gt1/gt1, Lman1^−/−, and Lman1^+/+ mice were analyzed by western blotting using an anti-A1AT antibody. The lower A1AT band corresponds to ER-retained A1AT (and was previously shown to be endoglycosidase H sensitive²⁸ ). The upper A1AT band corresponds to post-ER A1AT and is comparable in molecular weight to secreted plasma A1AT. Each lane corresponds to an individual mouse. (B) The ratio of post-ER A1AT:ER-retained A1AT (upper band:lower band) in the hepatic lysates of each mouse as determined by densitometry; a ratio >1 indicates that the majority of the A1AT has been secreted beyond the ER, whereas a ratio <1 indicates that the majority of the A1AT is retained in the ER. Horizontal lines indicate the mean, and error bars indicate the standard error of the mean for each genotype.