Iron overload improved upon treatment with iron chelator in CEP mice. Plasma iron (A), transferrin (B), transferrin saturation (%) (C), and ferritin (D) were determined in WT and CEP mice treated with DEF 1 or 3 mg/mL on euthanasia (26 weeks) using a multiparametric Olympus AU400 analyzer. (□) Individual mice. (E) Representative Perls staining of liver, spleen and kidney histologic sections from WT, CEP, and CEP mice treated with DEF 3 mg/mL at euthanasia (26 weeks). Many iron deposits (blue staining) were visible in liver macrophages, spleen tissue, and proximal convoluted tubules of the kidney cortex in control CEP mice compared with WT or CEP mice treated with DEF 3 mg/mL. Histologic sections were analyzed with a Nikon Eclipse Ni microscope (×20). Scale bar, 100 μM. Pictures were captured by using a digital camera (Nikon DS Ri2) and analyzed with NIS Elements AR software.