Activation of RET by GDNF/GFRα1 alters kinome dynamics during HSPC outgrowth. Heatmaps depicting serine/threonine (A) and tyrosine (B) containing row z-normalized peptide phosphorylations supervised by day and treatment. Rows are clustered by correlation. (C) Fold change differential phosphorylation of GDNF/GFRα1–treated CD34⁺CD38^– cells compared with control after 1 day of culture. (D) Upstream kinases calculated as responsible for phosphorylations in panel C. (E) Fold change differential phosphorylation of GDNF/GFRα1–treated CD34⁺CD38^– cells compared with control after 3 days of culture. (F) Upstream kinases calculated as responsible for phosphorylations in panel G. Red dots in panels C-F indicate significantly upregulated peptides or kinases; blue dots represent significantly downregulated peptides or kinases in response to GDNF/GFRα1 treatment.