GDNF/GFRα1 treatment induces anti-apoptotic and anti-inflammatory processes in cultured HSPCs. (A) Enriched process networks from significantly changed peptides in GDNF/GFRα1 vs control (Ctrl) cultures after 1 day (left, red) or 3 days (right, mustard). (B) Venn diagram depicting overlap of significantly altered peptides between day 1 (red) and day 3 (mustard) from GDNF/GFRα1 vs Ctrl cultures. (C) String protein network for differential phosphorylation events at day 1. Lines indicate reported interactions. (D-I) Key differential phosphorylations induced by GDNF/GFRα1 treatment at day 1, represented as relative phosphorylation. A Student t test was used to measure significant differences. Day 0 CD34⁺CD38^– input cells (gray), Ctrl (steel blue), and GDNF/GFRα1 (red) treatments at days 1 and 3 are presented. (J) String protein network for differential phosphorylation events at day 3. Lines indicate reported interactions. (K-M) Key differential phosphorylations induced by GDNF/GFRα1 treatment at day 3, represented as relative phosphorylation. For all graphs, a Student t test was used to calculate significant differences. *P < .05 vs Ctrl. Day 0 CD34⁺CD38⁻ input cells (gray), Ctrl (steel blue), and GDNF/GFRα1 (red) treatments at days 1 and 3 are presented.