Figure 6.
HSCs exhibit specific responses to GDNF/GFRα1 resulting in reduced accumulation of intracellular ROS. (A) Bar graphs depict median intensity of signal from histograms below illustrating profiles of key protein changes in HSCs at day 0 (blue), day 3 control (Ctrl; orange), day 3 GDNF/GFRα1 (green), day 7 Ctrl (red), and day 7 GDNF/GFRα1 (purple, a.u. = arbitrary units). (B) z-normalized heatmap illustrating differences in HSC clusters at input, and day 3 and day 7 culture with or without GDNF/GFRα1 treatment assayed by mass cytometry, supervised by treatment condition. (C) Mean fluorescence intensity of intracellular ROS in HSCs at day 7 ± GDNF/GFRα1/PZ1. (D) Histograms illustrating changes in intracellular ROS at day 7. (E) Percentage of HSCs in cultured cells at day 7 ± GDNF/GFRα1/PZ1. For panels C-E, *P < .05, N = 4.

HSCs exhibit specific responses to GDNF/GFRα1 resulting in reduced accumulation of intracellular ROS. (A) Bar graphs depict median intensity of signal from histograms below illustrating profiles of key protein changes in HSCs at day 0 (blue), day 3 control (Ctrl; orange), day 3 GDNF/GFRα1 (green), day 7 Ctrl (red), and day 7 GDNF/GFRα1 (purple, a.u. = arbitrary units). (B) z-normalized heatmap illustrating differences in HSC clusters at input, and day 3 and day 7 culture with or without GDNF/GFRα1 treatment assayed by mass cytometry, supervised by treatment condition. (C) Mean fluorescence intensity of intracellular ROS in HSCs at day 7 ± GDNF/GFRα1/PZ1. (D) Histograms illustrating changes in intracellular ROS at day 7. (E) Percentage of HSCs in cultured cells at day 7 ± GDNF/GFRα1/PZ1. For panels C-E, *P < .05, N = 4.

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