VAFs for TP53, BRCA1, and BRCA2 and over time in patients with hematopoietic malignancies. VAFs of DNA alterations in TP53 (A), BRCA1 (B), and BRCA2 (C) in individual patients at the University of Chicago are graphed over time. Each point indicates an individual variant identified in an in-house NGS assay, and red lines connect likely somatic variants; likely germline variants are shown in blue.