QPD results in a loss of repressive chromatin and repositions PLAU relative to a candidate megakaryocyte enhancer. (A) Genome browser view of tracks produced by ChIP-seq of H3K27ac (blue), H3K4me2 (orange), and H3K36me3 (red) in granulocytes and megakaryocytes, from QPD and control (CTL), and H3K27me3 (gray) and CTCF (green) from QPD and CTL megakaryocytes only (bottom). Each track shows merged signal from 3 biological replicates with the exception of the CTL granulocyte H3K4me2, for which only 2 biological replicates were used. ChIP-seq track values are shown as fold change over input. Arrowheads mark the position of ENH_QPD. The schematic (top) describes the duplication in QPD. Dashed lines mark the boundaries of the duplicated region. Tracks were visualized using the WashU epigenome browser with the following settings: summary method, max; smoothing window, 3 pixels. (B) ChIP-seq signal from QPD megakaryocytes and granulocytes aligned to a custom chromosome 10 containing the QPD duplication. Tracks were smoothed before visualization with Sushi⁷⁰  in R. Only unmapped reads and reads mapping to chromosome 10 from hg19 alignments were used.