Figure 6.
Schematic overview of [13C5,15N2]glutamine stable isotope tracings. (A) Tracing using [13C5,15N2]glutamine includes glutaminolytic and Krebs cycle pathways before (time 0) and 5 minutes after incubation with 1 µM fMLF. (B) Tracing using [13C5,15N2]glutamine in control and patient neutrophils before and after treatment with fMLF. The isotopologue intensities plotted correspond to labeling indicated in panel A (eg, 13C5,15N2 for Gln).