CD11c deficiency impairs HPSCs in the experimental polymicrobial abdominal sepsis model. Both WT and CD11c^−/− mice were subjected to polymicrobial abdominal sepsis (ASM) induced by cecal ligation and puncture surgery. (A) Sepsis survival. Shown is 1 of 2 independent experiments with the same pattern. (B) Blood leukocyte counts at 36 hours after surgery. (C) Sera collected before or at 12 hours after surgery was measured for G-CSF by ELISA. (D) Bone marrow (BM) B-cell, preneutrophil, and mature neutrophil counts at 36 hours after surgery. Preneutrophils were identified as Lin⁻CD115⁻SiglecF⁻Gr-1⁺CD11b⁺CXCR4^hic-kit^intCXCR2⁻ and mature neutrophils were identified as Lin⁻CD115⁻SiglecF⁻Gr-1⁺CD11b⁺CXCR4⁻c-kit⁻CXCR2⁺.⁶⁶  (E) Representative flow cytometry plot of LSKs and CMPs identified by costaining c-kit and sca-1 in lineage-negative BM cells. (F) Absolute number of LSKs and CMPs in 2 femurs of WT and CD11c^−/− mice at 24 hours after surgery. (G) Proliferation profile. At 20 hours after surgery, mice were pulsed with BrdU for an additional 4 hours, then analyzed for BrdU incorporation in the BM cells. (B-D,F-G) Symbols indicate individual mice. Data are representative of 2 experiments. Error bars indicate ± standard error of the mean (SEM). Statistical significance was tested with the log-rank test (A), Student t test (B,D,F-G), and 1-way analysis of variance (ANOVA) with Bonferroni post hoc analysis (C). *P < .05; **P < .01.