The expression of CD11c on HPSCs and its role. (A) Representative flow cytometry plot of LSKs and CMPs identified by costaining of c-kit and sca-1 in lineage-negative BM cells from naive WT and CD11c^−/− mice. (B) Absolute numbers of LSKs, CMPs, and CLPs in 2 femurs of naive WT and CD11c^−/− mice. (C) Representative flow cytometry plot profiling LT-HSC, ST-HSC, and MPP subpopulations in LSKs. (D) Absolute numbers of LT-HSCs, ST-HSCs, and MPPs in 2 femurs of WT and CD11c^−/− mice. (E) CD11c expression on lineage-negative BM cells confirmed by using CD11c^−/− mice. Shown was the representative histogram overlay analysis. BM cells from 3 WT or CD11c^−/− mice were pooled and used in each experiment; at least 5 independent experiments were done with the same pattern. (F) CD11c expression on LSKs, CMPs, CLPs, LT-HSCs, ST-HSCs, and MPPs in WT mice. Representative histograms are shown. (G) BrdU incorporation assay. Left, Representative histogram overlay of BrdU staining. Right, Cumulative analysis of frequency of BrdU⁺ population in WT and CD11c^−/− mice. (H) Volcano plot from RNA-sequencing analysis of LSK cells isolated from naive WT and CD11c^−/− mice. Data were representative of 5 experiments (A-F) or 3 experiments (G-H) with the same pattern. Symbols indicate individual mice. Error bars indicate ± SEM. (B,D,G) Statistical significance was tested by the Student t test.