SARS-CoV-2 epitope mapping of CSTs. T-cell epitope mapping of structural proteins was performed using minipools containing 8 to 24 peptides each, with responses measured via IFN-γ ELISpot (SFC per 1 × 10⁵ cells). (A) Epitopes within membrane protein were identified within the C terminus at AA 144-163 and 173-192, which were recognized by 8 and 6 donors, respectively. (B) Mapping of spike epitopes demonstrated three regions at AA 57-75, 205-224, and 449-463, which were recognized by 3 donors. (C) Mapping of nucleocapsid epitopes showed 2 regions at AA 357-271 and 313-335 were recognized by 3 donors.