N- and O-linked glycosylation of human VWF. (A) Before secretion from ECs, VWF undergoes complex posttranslational modification that includes significant N- and O-linked glycosylation. (B) Each VWF monomer contains 13 N- and 10 O-linked glycan chains. (C) Complex biantennary, triantennary, and tetra-antennary chains are expressed on the N-glycans of VWF. In contrast, core 1, core 2, and disialosyl structures are common on the O-glycans.