Pretreatment with siFXIIIB renders arterial thrombi more susceptible to lysis in vivo. Doppler ultrasound measured blood flow after thrombi were induced in the carotid artery with a 10% w/v solution of FeCl₃, and tenecteplase was administered at a dose of 9 mg/kg. (A-B) Representative Doppler graphs show the time when FeCl₃ was applied (black arrow, followed by a 2-minute gap in measurement), occlusion of the vessel, drop in blood flow, injection of tenecteplase (light blue arrow), and then either no recovery of blood flow (A, siLuc treated) or stable reperfusion (B, siFXIIIB treated). (C) Quantification of panels A and B, measuring the blood flow until 60 minutes after occlusion (n = 9). Data markers indicate whether reperfusion was stable (green), transient (red), or if no reperfusion occurred (white). For all graphs, values represent mean ± SEM. ns, P > .05; **P < .01.