Gene-expression profile and TCR repertoire development during anti-PD1 treatment. (A) WSI of CD8 in paired samples (ns, 2-tailed paired Student t test). Red line, mean value. (B) Gene expression for cytotoxicity. *P < .05, Student t test. (C-E) TCR repertoire analysis of paired samples from 4 patients. (C) Productive clonality (1 minus normalized Shannon’s entropy) in paired samples (P value = ns, 2-tailed paired Student t test). (D) Percentage of the 10 most frequent clones in the total repertoire in each sample. (E) Scatterplot of productive TCR rearrangement in 5 paired biopsies. A total of 238 of 12 442 TCRs were shared between the time points. Shared TCRs that differ significantly between both time points (P < .05, Fisher’s test) are colored red. LN, lymph node; ns, not significant.