Single-cell transcriptome analysis reveals clusters of naïve and lineage-committed hematopoietic progenitors. (A) Schematic of the scRNAseq experiment where iPSCs (SFCi55) were differentiated in vitro (IVD) for 13 days (supplemental Figure 1A); CD235a⁻CD43⁺ suspension cells were isolated by flow cytometry and subjected to a 10x Genomics sequencing platform. (B) tSNE visualization of 11 420 cells divided into 8 clusters, including those defined by gene expression as naïve (naïve 1 and 2), and others that expressed genes associated with erythroid (Ery 1 and 2), megakaryocyte (Mega 1, 2, and 3), and granulocyte (Granulo) lineages. (C) Heatmap showing expression of the top 10 marker genes for each cluster (colors of each cluster are as in panel B). (D) Gene expression levels of marker genes associated with different progenitor cell types that were identified by clustering, visualized on tSNE. tSNE, t-distributed stochastic neighbor embedding.