Differential gene expression and pathway analysis demonstrate the immune phenotype of adult lung MKs. Heat map and gene set enrichment analysis illustrating robust upregulation of immune/inflammatory genes in adult lung MKs. Adult BM cluster 1 showed enrichment for processes important in proplatelet production, and this gene set was notably also upregulated in the adult lung population (dashed black box). The top 20 differentially expressed genes associated with each cluster are listed to the left of the heat map. The gene ontology pathways associated with the remaining clusters are shown in supplemental Figure 7.